Accuracy of interleukin-27 in diagnosing tuberculous pleural effusion: Age should be considered.

IF 3.7 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Cytokine Pub Date : 2025-02-01 Epub Date: 2024-12-31 DOI:10.1016/j.cyto.2024.156844

Hong-Zhe Zhu, Yan Niu, Jian-Xun Wen, Cheng Yan, Su-Na Cha, Yue Gao, Xu-Lei Hao, Wen-Jie Hou, Li Yan, Ting-Wang Jiang, Zhi-De Hu, Wen-Qi Zheng

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引用次数: 0

Abstract

Background: Tuberculous pleural effusion (TPE) diagnosis still faces many difficulties and challenges. Some studies have shown that pleural interleukin -27 (IL-27) had a diagnostic potential for TPE. However, their findings are not always consistent. This study aimed to investigate the diagnostic accuracy of pleural IL-27 for TPE.

Methods: We prospectively enrolled 211 patients with undiagnosed pleural effusion. Effusion Mycobacterium tuberculosis (Mtb) culture, Ziehl-Neelsen staining, biopsy, and response to antituberculosis therapy were used to define TPE. The pleural IL-27 levels were determined by enzyme-linked immunosorbent assay (ELISA). A receiver operating characteristic curve (ROC) with the area under the curve (AUC) was used to evaluate the diagnostic accuracy of IL-27 for TPE. In addition, we investigated the influence of age on the diagnostic performance of IL-27 by resampling patients with different upper age limits in the inclusion criteria.

Results: Among the 211 enrolled participants, 33 were TPE and 178 were non-TPE. The mean concentration of IL-27 in TPE patients was significantly higher than that of non-TPE patients. The AUC of IL-27 was 0.76 (95 %CI: 0.67-0.86). At the threshold of 500 pg/mL, the sensitivity and specificity of IL-27 were 0.26 (95 %CI: 0.20-0.33) and 0.91 (95 %CI:0.76-0.97), respectively. The AUC of IL-27 is 0.84 in patients with an upper age limit of 70. Still, it decreased to 0.76 in patients with an upper age limit of 75.

Conclusion: Age can affect the diagnostic performance of IL-27 for TPE.

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白细胞介素-27诊断结核性胸腔积液的准确性：应考虑年龄。

背景：结核性胸腔积液（TPE）的诊断仍面临许多困难和挑战。一些研究表明，胸膜白介素-27 （IL-27）有诊断TPE的潜力。然而，他们的发现并不总是一致的。本研究旨在探讨胸膜IL-27对TPE的诊断准确性。方法：我们前瞻性地纳入211例未确诊的胸腔积液患者。结核分枝杆菌（Mtb）积液培养、Ziehl-Neelsen染色、活检和对抗结核治疗的反应被用来定义TPE。采用酶联免疫吸附试验（ELISA）检测胸膜IL-27水平。采用受试者工作特征曲线（ROC）和曲线下面积（AUC）评价IL-27对TPE的诊断准确性。此外，我们通过重新抽样纳入标准中不同年龄上限的患者，研究年龄对IL-27诊断效能的影响。结果：211名受试者中，TPE患者33名，非TPE患者178名。TPE患者IL-27的平均浓度明显高于非TPE患者。IL-27的AUC为0.76 （95% CI: 0.67 ~ 0.86）。在500 pg/mL阈值下，IL-27的敏感性和特异性分别为0.26 （95% CI: 0.20 ~ 0.33）和0.91 （95% CI:0.76 ~ 0.97）。年龄上限为70岁的患者IL-27的AUC为0.84。然而，在年龄上限为75岁的患者中，它降至0.76。结论：年龄会影响IL-27对TPE的诊断效能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cytokine 医学-免疫学

CiteScore

7.60

自引率

2.60%

发文量

262

审稿时长

48 days

期刊介绍： The journal Cytokine has an open access mirror journal Cytokine: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. * Devoted exclusively to the study of the molecular biology, genetics, biochemistry, immunology, genome-wide association studies, pathobiology, diagnostic and clinical applications of all known interleukins, hematopoietic factors, growth factors, cytotoxins, interferons, new cytokines, and chemokines, Cytokine provides comprehensive coverage of cytokines and their mechanisms of actions, 12 times a year by publishing original high quality refereed scientific papers from prominent investigators in both the academic and industrial sectors. We will publish 3 major types of manuscripts: 1) Original manuscripts describing research results. 2) Basic and clinical reviews describing cytokine actions and regulation. 3) Short commentaries/perspectives on recently published aspects of cytokines, pathogenesis and clinical results.