Application of Carbon Quantum Dots Derived from Waste Tea for the Detection of Pesticides in Tea: A Novel Biosensor Approach.

Abstract

Chemical pesticide residues have negative consequences for human health and the environment. Prioritizing a detection method that is both reliable and efficient is essential. Our innovative research explored the application of biosensors based on carbon quantum dots (CQDs) derived from waste tea to detect commonly used pesticides in tea. CQDs have been synthesized using a simple one-pot hydrothermal approach and thoroughly characterized using advanced techniques such as high-resolution transmission electron microscopy, ultraviolet-visible spectroscopy, photoluminescence (PL) spectroscopy, Raman spectroscopy, X-ray diffraction, atomic force microscopy, and X-ray photoelectron spectroscopy. The fluorescence resonance energy transfer-based fluorescence "turn on-off" mechanism has been successfully employed to study the detection of four different pesticides, viz., quinalphos 25 EC, thiamethoxam 25 WG, propargite 57 EC, and hexaconazole 5 EC. The detection limits for quinalphos 25 EC, thiamethoxam 25 WG, and propargite 57 EC were determined to be 0.2, 1, and 10 ng/mL, respectively. Notably, these values are significantly lower than the maximum residue level for each pesticide. We achieved a strong linear correlation (R = -0.96) with a detection limit of 0.2 ng/mL for quinalphos 25 EC. The quantum yield was determined to be 40.05%. Our research demonstrates that the developed nanobiosensor reliably and accurately detects pesticides, including those present in experimental samples containing mixtures of pesticides.