Protective and haemato-immunological effect of seaweed crude extracts against Tilapia Lake Virus (TiLV) in Nile tilapia, Oreochromis niloticus

IF 2.2 3区农林科学 Q2 FISHERIES

Aquaculture International Pub Date : 2025-01-02 DOI:10.1007/s10499-024-01795-8

Kaviyarasu Periyannan, Sivasankar Panchavarnam, Chrisolite Bagthasingh, Subash Palaniappan, Priyadharshini Michael, Mageshkumar Palraj, Mohamed Mansoor Mohaideenpitchai, Selvamagheswaran Muthumariyapan

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引用次数: 0

Abstract

Stimulation of non-specific immune responses in Nile tilapia by seaweed-based natural compounds is a potential solution for the deterrence of Tilapia Lake Virus (TiLV) outbreaks. The present study investigated the protective and immunostimulatory effects of different seaweed extracts against TiLV. The study deals with hot water extracts of ten seaweeds in total namely Gracilaria edulis, Ulva fasciata, Caulerpa toxifolia, Turbinaria ornata, Enteromorpha compressa, Sargassum wightii, Sargassum ilicifolium, Sargassum muticum, Hynea musciformis and Chaetomorpha aerea collected from the Gulf of Mannar coast of Mandapam and Rameswaram, Tamil Nadu. Among these, extracts of three seaweeds such as S. ilicifolium, U. fasciata, and E. compressa exhibited notable antiviral effects by inhibiting cytopathic effect without cell toxicity in striped snakehead (SSN1) cell line. These seaweed extracts were further incorporated into artificial pellet feed at different concentrations (100, 300 and 500 mg/kg). Of which, tilapia fed with diet containing S. ilicifolium at 500 mg/kg of feed showed the highest RPS value (58.34%) followed by U. fasciata (50%) and E. compressa (41.67%). Additionally, significant (P < 0.05) improvements in haematological parameters, immunological parameters and immune gene expression were observed in the S. ilicifolium-fed group at 24, 48 and 72 h post-infection. The findings highlight S. ilicifolium extract as a natural dietary immunostimulant that enhances the health, productivity and management of TiLV in tilapia farming, promoting a sustainable approach to mitigate disease outbreaks in commercial aquaculture.

查看原文本刊更多论文

海藻粗提物对尼罗罗非鱼（Oreochromis niloticus）抗罗非湖病毒（TiLV）的保护和血液免疫作用

以海藻为基础的天然化合物刺激尼罗罗非鱼的非特异性免疫反应是遏制罗非鱼湖病毒（TiLV）爆发的潜在解决方案。本研究探讨了不同海藻提取物对TiLV的保护作用和免疫刺激作用。本研究共提取了10种海藻的热水提取物，分别是毛茛属（Gracilaria edulis）、筋膜藻属（Ulva fasciata）、毒叶藻属（Caulerpa toxifolia）、鸟尾藻属（Turbinaria ornata）、压缩Enteromorpha compressa、马尾藻（Sargassum wightii）、马尾藻（Sargassum ilicifolium）、马尾藻（Sargassum muticum）、海鞘（Hynea musciformis）和毛羽藻（Chaetomorpha aerea），这些海藻采集自Mandapam和Rameswaram、泰米尔纳德邦的Mannar湾海岸。其中，三种海藻（S. ilicifolium， U. fasciata， E. compressa）提取物对条纹蛇头（SSN1）细胞株具有明显的抗病毒作用，可抑制细胞病变而无细胞毒性。将这些海藻提取物以不同浓度（100、300和500 mg/kg）添加到人工颗粒饲料中。其中，在500 mg/kg饲料水平下，罗非鱼的RPS值最高（58.34%），其次是筋膜棘鱼（50%）和扁棘鱼（41.67%）。此外，在感染后24、48和72 h，喂养组的血液学参数、免疫学参数和免疫基因表达均有显著改善（P < 0.05）。这些发现强调了S. ilicifolium提取物作为一种天然的膳食免疫刺激剂，可以提高罗非鱼养殖中TiLV的健康、生产力和管理，促进一种可持续的方法来减轻商业水产养殖中的疾病暴发。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Aquaculture International 农林科学-渔业

CiteScore

5.10

自引率

6.90%

发文量

204

审稿时长

1.0 months

期刊介绍： Aquaculture International is an international journal publishing original research papers, short communications, technical notes and review papers on all aspects of aquaculture. The Journal covers topics such as the biology, physiology, pathology and genetics of cultured fish, crustaceans, molluscs and plants, especially new species; water quality of supply systems, fluctuations in water quality within farms and the environmental impacts of aquacultural operations; nutrition, feeding and stocking practices, especially as they affect the health and growth rates of cultured species; sustainable production techniques; bioengineering studies on the design and management of offshore and land-based systems; the improvement of quality and marketing of farmed products; sociological and societal impacts of aquaculture, and more. This is the official Journal of the European Aquaculture Society.