Gynogenesis in the spotted catfish Pimelodus maculatus using heterologous gametes from the yellowtail-tetra (Astyanax altiparanae): a key point to achieve monosex female populations in Neotropical catfishes

IF 2.2 3区农林科学 Q2 FISHERIES

Aquaculture International Pub Date : 2025-01-02 DOI:10.1007/s10499-024-01785-w

Rafaela Manchin Bertolini, Lucia Suárez López, Bruna dos Santos Machado, Nivaldo Ferreira do Nascimento, José Augusto Senhorini, Norberto Castro Vianna, George Shigueki Yasui

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Abstract

Monosex populations in aquaculture is important to increase performance for subsequent food supply. Gynogenesis is the main tool to achieve monosex population within only one generation, since it provides only maternal inheritance. The present study aimed to achieve gynogenetic individuals of spotted catfish (Pimelodus maculatus), an important Neotropical species in which the female presents better growth performance. Sperm of yellowtail-tetra (Astyanax altiparanae) was UV-irradiated (80 mJ/cm²) in order to inactivate paternal genome. Afterwards, oocytes of P. maculatus were fertilized with the irradiated sperm and heat-shocked (38 °C for 2 min, 2 mpf) for diploidization by prevention of the release of the 2nd polar body. Diploid progenies derived from P. maculatus couples served as controls, and a non-treated haploid was also produced. Flow cytometric analysis and chromosome number obtained by cytogenetic analysis confirmed that gynogenetic individuals had the same number of chromosomes as the control group, indicating that sperm inactivation and diploidization was successful. Genotyping by microsatellite markers confirmed the maternal inheritance in these gynogenetics. Macroscopic visualization of the gonads revealed an increased number of females (n = 48) and only two males, suggesting a homogametic sex determination system (XX/XY). Finally, an efficient protocol for inducing meiotic gynogenesis in P. maculatus was established using irradiated sperm from A. altiparanae. This protocol can apply to produce monosex population and clonal lines in the P. maculatus species and can contribute for future studies in basic and applied sciences.

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利用黄尾四目鱼的异源配子进行斑点鲶鱼雌体发育：新热带鲶鱼实现单性雌性种群的关键

水产养殖中的单性种群对提高后续食物供应的生产性能具有重要意义。雌性生殖是在一代内实现单性群体的主要工具，因为它只提供母系遗传。斑点鲶鱼（Pimelodus maculatus）是一种重要的新热带物种，雌性的生长性能较好，本研究旨在获得雌性的个体。用80 mJ/cm2紫外线照射黄尾四鱼精子，使其父系基因组失活。然后，用辐照后的精子使斑马鱼卵母细胞受精，并通过阻止第二极体的释放进行热休克（38°C, 2min, 2mpf）进行二倍体分化。以斑马鱼夫妇的二倍体后代作为对照，也产生了未处理的单倍体。流式细胞术分析和细胞遗传学分析获得的染色体数目证实，雌核发生个体的染色体数目与对照组相同，表明精子失活和二倍体化成功。微卫星标记基因分型证实了母系遗传。性腺的宏观可视化显示雌性数量增加（n = 48），而雄性只有2只，表明性别决定系统是同质的（XX/XY）。最后，建立了一种有效的诱导黄斑鱼减数分裂雌核发生的方法。该方法可用于黄斑藤单性群体和无性系的生产，为今后在基础科学和应用科学方面的研究做出贡献。

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来源期刊

Aquaculture International 农林科学-渔业

CiteScore

5.10

自引率

6.90%

发文量

204

审稿时长

1.0 months

期刊介绍： Aquaculture International is an international journal publishing original research papers, short communications, technical notes and review papers on all aspects of aquaculture. The Journal covers topics such as the biology, physiology, pathology and genetics of cultured fish, crustaceans, molluscs and plants, especially new species; water quality of supply systems, fluctuations in water quality within farms and the environmental impacts of aquacultural operations; nutrition, feeding and stocking practices, especially as they affect the health and growth rates of cultured species; sustainable production techniques; bioengineering studies on the design and management of offshore and land-based systems; the improvement of quality and marketing of farmed products; sociological and societal impacts of aquaculture, and more. This is the official Journal of the European Aquaculture Society.