Targeted delivery of chrysin and 5-fluorouracil on MDA-MB-231 cancer cells by a peptide-functionalized L-DOPA-imprinted polymer.

IF 3.1 4区 医学 Q2 PHARMACOLOGY & PHARMACY
Sedighe Yosefi, Majid Sirati-Sabet, Abbas Pakdel, Zahra Nabizadeh, Parviz Kokhaei, Hamid Madanchi
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引用次数: 0

Abstract

Triple-negative breast cancer (TNBC) is a very aggressive and deadly form of breast cancer for which chemotherapy is the only systemic treatment option. Therefore, novel and more effective targeted or combined therapies, such as specific drug delivery systems that selectively target cancer cells, have received much attention. This research aimed to investigate the effect of targeted delivery of chrysin (CH) and 5-fluorouracil (5FU) using polymer nanoparticles on MDA-MB-231 cells. In this regard, CH and 5FU were individually used as the template to polymerize L-DOPA on the surface of silica nanoparticles. Then, a CD138-targeting peptide was designed for the first time and immobilized on the surface of the polymeric nanocomposite to target TNBC. The results showed that poly(L-DOPA)-CH-peptide and poly(L-DOPA)-5FU-peptide are selective for MDA-MB-231 cells and deliver drugs to them in a targeted manner. In this study, peptide-containing nanocomposites targeting CD138 were more successful in reducing cell proliferation than peptide-free nanocomposites. Also, they increased apoptosis and cell cycle arrest in MDA-MB-231 cancer cells in vitro. The effective and targeted delivery of CH and 5FU to MDA-MB-231 cancer cells by the designed interference peptide in this study can promise an effective treatment method for inhibiting the growth and progression of cancer. However, animal studies are needed to understand the efficacy of the interfering peptide and the final designed construct.

一种多肽功能化的左旋多巴印迹聚合物靶向递送菊花素和5-氟尿嘧啶至MDA-MB-231癌细胞。
三阴性乳腺癌(TNBC)是一种非常具有侵袭性和致命性的乳腺癌,化疗是唯一的全身治疗选择。因此,新的和更有效的靶向或联合治疗,如选择性靶向癌细胞的特异性药物输送系统,受到了广泛的关注。本研究旨在探讨聚合物纳米颗粒靶向递送金菊素(CH)和5-氟尿嘧啶(5FU)对MDA-MB-231细胞的影响。为此,分别以CH和5FU作为模板在二氧化硅纳米颗粒表面聚合L-DOPA。然后,首次设计了cd138靶向肽,并将其固定在聚合物纳米复合材料表面以靶向TNBC。结果表明,poly(L-DOPA)-CH-peptide和poly(L-DOPA)-5FU-peptide对MDA-MB-231细胞具有选择性,可靶向给药。在这项研究中,靶向CD138的含肽纳米复合材料比不含肽的纳米复合材料更能成功地减少细胞增殖。此外,它们还能增加MDA-MB-231癌细胞的凋亡和细胞周期阻滞。本研究设计的干扰肽可将CH和5FU有效靶向递送至MDA-MB-231癌细胞,有望成为抑制肿瘤生长和进展的有效治疗方法。然而,需要动物实验来了解干扰肽的功效和最终设计的结构。
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来源期刊
CiteScore
6.20
自引率
5.60%
发文量
142
审稿时长
4-8 weeks
期刊介绍: Naunyn-Schmiedeberg''s Archives of Pharmacology was founded in 1873 by B. Naunyn, O. Schmiedeberg and E. Klebs as Archiv für experimentelle Pathologie und Pharmakologie, is the offical journal of the German Society of Experimental and Clinical Pharmacology and Toxicology (Deutsche Gesellschaft für experimentelle und klinische Pharmakologie und Toxikologie, DGPT) and the Sphingolipid Club. The journal publishes invited reviews, original articles, short communications and meeting reports and appears monthly. Naunyn-Schmiedeberg''s Archives of Pharmacology welcomes manuscripts for consideration of publication that report new and significant information on drug action and toxicity of chemical compounds. Thus, its scope covers all fields of experimental and clinical pharmacology as well as toxicology and includes studies in the fields of neuropharmacology and cardiovascular pharmacology as well as those describing drug actions at the cellular, biochemical and molecular levels. Moreover, submission of clinical trials with healthy volunteers or patients is encouraged. Short communications provide a means for rapid publication of significant findings of current interest that represent a conceptual advance in the field.
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