Metal-Mediated Protein Engineering within Live Cells

IF 3.5 3区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Chemistry - An Asian Journal Pub Date : 2024-12-30 DOI:10.1002/asia.202401669

Dr. Pritam Ghosh

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Abstract

Metal mediated several organic reactions are known which can be used inside the cellular medium for protein modifications, eventually for targeting diseases. Indeed, due to their ease of handling, rapid solubility, and effective cell penetration, metals are superior than any other competitor as a stimulus/mediator in organic reactions relevant with protein modifications. Metal mediated most effective reactions as a chemical biology tool are Cu(I)-catalyzed azide-alkyne cycloaddition(CuAAC)/click reactions or Pd mediated multiple chemical reactions for intra/extra cellular protein modifications etc. A few examples of Au(III), Ru(III) are also known. Among these, the click reaction has high potential for the management of biomolecules within cells, and thus this methodology is adopted broadly in chemistry, biology towards therapeutic applications in pharmacology. Fast kinetics in aqueous medium at ambient to normal temperature with specificity between precursors (e. g., azide and alkyne for click reactions which are bio-orthogonal to cells) are essential aspects behind the success of metal mediated intracellular reactions. This review dealt with specifically metal mediated protein modifications within live cells, the achievements and challenges.

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活细胞内金属介导的蛋白质工程。

已知金属介导的几种有机反应可以在细胞介质中用于蛋白质修饰，最终用于靶向疾病。事实上，由于易于处理-快速溶解度-快速细胞渗透，金属作为与蛋白质修饰相关的有机反应的刺激/介质优于任何其他竞争对手。金属介导的最有效的化学生物学工具是Cu(I)催化的叠氮-炔环加成(CuAAC)/click反应或Pd介导的细胞内/细胞外蛋白质修饰的多重化学反应等。Au（III）、Ru（III）也有几个例子。其中，点击反应对细胞内生物分子的管理具有很高的潜力，因此这种方法在化学、生物学中被广泛采用，并在药理学中得到治疗应用。在水介质中，在室温到常温下，前体（例如，叠氮化物和炔烃的生物正交反应）之间的快速动力学是金属介导的细胞内反应成功背后的重要方面。本文综述了活细胞内金属介导的特异性蛋白修饰、成就和挑战。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chemistry - An Asian Journal 化学-化学综合

CiteScore

7.00

自引率

2.40%

发文量

535

审稿时长

1.3 months

期刊介绍： Chemistry—An Asian Journal is an international high-impact journal for chemistry in its broadest sense. The journal covers all aspects of chemistry from biochemistry through organic and inorganic chemistry to physical chemistry, including interdisciplinary topics. Chemistry—An Asian Journal publishes Full Papers, Communications, and Focus Reviews. A professional editorial team headed by Dr. Theresa Kueckmann and an Editorial Board (headed by Professor Susumu Kitagawa) ensure the highest quality of the peer-review process, the contents and the production of the journal. Chemistry—An Asian Journal is published on behalf of the Asian Chemical Editorial Society (ACES), an association of numerous Asian chemical societies, and supported by the Gesellschaft Deutscher Chemiker (GDCh, German Chemical Society), ChemPubSoc Europe, and the Federation of Asian Chemical Societies (FACS).