Multimodal cadmium resistance and its regulatory networking in Pseudomonas aeruginosa strain CD3.

IF 3.9 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Scientific Reports Pub Date : 2024-12-30 DOI:10.1038/s41598-024-80754-y

Soumya Chatterjee, Partha Barman, Chandan Barman, Sukanta Majumdar, Ranadhir Chakraborty

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Abstract

Cadmium, a toxic heavy metal, poses significant global concern. A strain of the genus Pseudomonas, CD3, demonstrating significant cadmium resistance (up to 3 mM CdCl₂.H₂O) was identified from a pool of 26 cadmium-resistant bacteria isolated from cadmium-contaminated soil samples from Malda, India. The minimum inhibitory concentrations (MICs) for cadmium and other heavy metals/metalloids were determined with clarity using a modified chemically-defined medium inoculated with variable inoculum density. Formation of biofilm enabled CD3 cells to resist up to 0.75 mM CdCl₂.H₂O. Survival and growth of CD3 cells in presence of > 1 mM CdCl₂.H₂O was dependent on efflux mechanism. Efflux mechanism in CD3 was confirmed by atomic absorption spectroscopy. Resistance to cadmium was inducible when grown in presence of ≥ 1.0 mM CdCl₂.H₂O. Minimum concentration of cadmium or zinc or cobalt salts required for induction of cadmium resistance was determined. Whole-genome-based phylogenetic tools identified CD3 as the closest relative to Pseudomonas aeruginosa DSM50071^T. Bioinformatic analyses revealed a complex network of regulations, with BfmR playing a crucial role in the functions of CzcR and CzcS, essential for biofilm formation and receptor signalling pathways. Comparative genomics and mutation landscape analyses of cadmium-resistance genes in P. aeruginosa strains revealed dynamism in evolution of cadmium resistance.

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铜绿假单胞菌CD3菌株的多模态镉抗性及其调控网络。

镉是一种有毒的重金属，引起了全球的严重关注。从印度Malda镉污染土壤样品中分离的26株抗镉细菌中鉴定出一株CD3假单胞菌属，显示出显著的镉抗性（高达3 mM CdCl2.H2O）。对镉和其他重金属/类金属的最低抑制浓度（mic）是用一种改良的化学定义培养基，接种可变接种密度，用透明法测定的。生物膜的形成使CD3细胞能够抵抗0.75 mM的CdCl2.H2O。CdCl2存在下CD3细胞的存活和生长。H2O依赖于外排机制。用原子吸收光谱法证实了CD3的外排机理。在≥1.0 mM的CdCl2.H2O环境下生长，对镉具有抗性。测定了诱导耐镉性所需的镉或锌或钴盐的最低浓度。基于全基因组的系统发育工具鉴定出CD3是铜绿假单胞菌DSM50071T的近亲。生物信息学分析揭示了一个复杂的调控网络，BfmR在CzcR和CzcS的功能中起着至关重要的作用，对生物膜的形成和受体信号通路至关重要。铜绿假单胞菌耐镉基因的比较基因组学和突变景观分析揭示了耐镉进化的动态性。

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来源期刊

Scientific Reports Natural Science Disciplines-

CiteScore

7.50

自引率

4.30%

发文量

19567

审稿时长

3.9 months

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