Antiviral activity of Ferula assa-feotida on HSV-1, 2 in vitro.

IF 1.3 Q4 MICROBIOLOGY

Iranian Journal of Microbiology Pub Date : 2024-12-01 DOI:10.18502/ijm.v16i6.17257

Javad Charostad, Tahere Navidfar, Masoumeh Kiani, Paul Schinitzler, Akram Astani

{"title":"Antiviral activity of Ferula assa-feotida on HSV-1, 2 in vitro.","authors":"Javad Charostad, Tahere Navidfar, Masoumeh Kiani, Paul Schinitzler, Akram Astani","doi":"10.18502/ijm.v16i6.17257","DOIUrl":null,"url":null,"abstract":"Background and objectives: Medicinal plants are the primary treatment for many infectious and non-infectious diseases. In this study, we evaluated the antiviral activity of Ferula assa-feotida against herpes simplex viruses 1 and 2, and compared it with the antiviral drug acyclovir.Materials and methods: In our experimental study, Ferula assa-feotida was dissolved in DMSO, then diluted in DMEM medium. Acyclovir was used at a concentration of 100 μM in all procedures. The antiherpetic activity and Antiviral activity of Ferula were evaluated in Vero cells (African green monkey kidney cells) by using the plaque reduction assay.Results: Inhibitory concentrations of 50% (IC50) of Ferula assa-feotida for HSV-1 and HSV-2 were determined at 0.00025% and 0.00015%, respectively. Ferula was introduced at various stages of viral infection and significantly inhibited HSV-1 and HSV-2 infectivity by > 95.5% and 89%, respectively, when virus was pre-treated before addition to the cells. No HSV-1 or HSV-2 activity was detected in cells treated prior to and following viral infection.Conclusion: These results indicate that Ferula assa-feotida demonstrates antiherpetic activity in the early phase of viral infection and could be used as potential antiviral agent.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"16 6","pages":"786-791"},"PeriodicalIF":1.3000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11682560/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18502/ijm.v16i6.17257","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background and objectives: Medicinal plants are the primary treatment for many infectious and non-infectious diseases. In this study, we evaluated the antiviral activity of Ferula assa-feotida against herpes simplex viruses 1 and 2, and compared it with the antiviral drug acyclovir.

Materials and methods: In our experimental study, Ferula assa-feotida was dissolved in DMSO, then diluted in DMEM medium. Acyclovir was used at a concentration of 100 μM in all procedures. The antiherpetic activity and Antiviral activity of Ferula were evaluated in Vero cells (African green monkey kidney cells) by using the plaque reduction assay.

Results: Inhibitory concentrations of 50% (IC₅₀) of Ferula assa-feotida for HSV-1 and HSV-2 were determined at 0.00025% and 0.00015%, respectively. Ferula was introduced at various stages of viral infection and significantly inhibited HSV-1 and HSV-2 infectivity by > 95.5% and 89%, respectively, when virus was pre-treated before addition to the cells. No HSV-1 or HSV-2 activity was detected in cells treated prior to and following viral infection.

Conclusion: These results indicate that Ferula assa-feotida demonstrates antiherpetic activity in the early phase of viral infection and could be used as potential antiviral agent.

查看原文本刊更多论文

阿魏对hsv - 1,2的体外抗病毒活性研究。

背景和目的：药用植物是许多传染性和非传染性疾病的主要治疗药物。在这项研究中，我们评估了阿魏对单纯疱疹病毒1和2的抗病毒活性，并将其与抗病毒药物阿昔洛韦进行了比较。材料与方法：在我们的实验研究中，阿魏在DMSO中溶解，然后在DMEM培养基中稀释。所有步骤均使用浓度为100 μM的阿昔洛韦。采用斑块减少法测定阿魏在非洲绿猴肾细胞中的抗疱疹活性和抗病毒活性。结果：阿魏对HSV-1和HSV-2的50%抑制浓度（IC50）分别为0.00025%和0.00015%。在病毒感染的不同阶段引入阿魏，在加入细胞前对病毒进行预处理，对HSV-1和HSV-2的传染性分别有显著的抑制作用，分别为95.5%和89%。在病毒感染前后处理的细胞中未检测到HSV-1或HSV-2活性。结论：阿魏在病毒感染早期具有抗疱疹活性，可作为潜在的抗病毒药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Iranian Journal of Microbiology MICROBIOLOGY-

CiteScore

2.40

自引率

7.10%

发文量

审稿时长

12 weeks

期刊介绍： The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.