Single-cell RNA sequencing identifies the expression of hemoglobin in chondrocyte cell subpopulations in osteoarthritis.

IF 2.4 3区 生物学 Q4 CELL BIOLOGY
Zhihao Zhang, Ting He, Hongwen Gu, Yuanhang Zhao, Shilei Tang, Kangen Han, Yin Hu, Hongwei Wang, Hailong Yu
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引用次数: 0

Abstract

In recent years, chondrocytes have been found to contain hemoglobin, which might be an alternative strategy for adapting to the hypoxic environment, while the potential mechanisms of that is still unclear. Here, we report the expression characteristics and potential associated pathways of hemoglobin in chondrocytes using single-cell RNA sequencing (scRNA-seq). We downloaded data of normal people and patients with osteoarthritis (OA) from the Gene Expression Omnibus (GEO) database and cells are unbiased clustered based on gene expression pattern. We determined the expression levels of hemoglobin in various chondrocyte subpopulations. Meanwhile, we further explored the difference in the enriched signaling pathways and the cell-cell interaction in chondrocytes of the hemoglobin high-expression and low-expression groups. Specifically, we found that SPP1 was closely associated with the expression of hemoglobin in OA progression. Our findings provide new insights into the distribution characteristics of hemoglobin in chondrocytes and provide potential clues to the underlying role of hemoglobin in OA and the mechanisms related to that, providing potential new ideas for the treatment of OA.

单细胞RNA测序鉴定骨关节炎软骨细胞亚群中血红蛋白的表达。
近年来,软骨细胞被发现含有血红蛋白,这可能是适应缺氧环境的另一种策略,但其潜在机制尚不清楚。在这里,我们使用单细胞RNA测序(scRNA-seq)报告了软骨细胞中血红蛋白的表达特征和潜在的相关途径。我们从Gene Expression Omnibus (GEO)数据库中下载了正常人和骨关节炎(OA)患者的数据,并根据基因表达模式对细胞进行了无偏聚类。我们测定了血红蛋白在不同软骨细胞亚群中的表达水平。同时,我们进一步探讨了血红蛋白高表达组和低表达组软骨细胞中富集的信号通路和细胞间相互作用的差异。具体来说,我们发现SPP1与OA进展中血红蛋白的表达密切相关。我们的发现对软骨细胞中血红蛋白的分布特征提供了新的见解,并为血红蛋白在OA中的潜在作用及其相关机制提供了潜在的线索,为OA的治疗提供了潜在的新思路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
BMC Molecular and Cell Biology
BMC Molecular and Cell Biology Biochemistry, Genetics and Molecular Biology-Cell Biology
CiteScore
5.50
自引率
0.00%
发文量
46
审稿时长
27 weeks
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