Identification of bla_OXA-51-23-58, bla_VIM, bla_NDM, and bla_IMP carbapenemase genes in Acinetobacter baumannii isolates from hospitalized patients.

IF 1.6 Q2 MULTIDISCIPLINARY SCIENCES

BMC Research Notes Pub Date : 2024-12-27 DOI:10.1186/s13104-024-07047-5

Kosar Salmani Nasrabadi, Yasaman Ahmadi, Seyed Mahdi Ghasemi, Dariush Shokri

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引用次数: 0

Abstract

Objective: The increase of multidrug-resistant (MDR) strains of Acinetobacter baumannii (A. baumannii), especially carbapenem-resistant strains, is challenging for treating infections. This study investigated the antibiotic resistance pattern and frequency of carbapenem resistance genes (oxacillinase and metallo-beta-lactamase) in A. baumannii.

Results: In this study, 100 bacterial isolates were collected from clinical samples from different hospitals in Isfahan, central of Iran. Of 100 samples of bloodstream, urine, cerebrospinal fluid (CSF), wound, and trachea, 60 bacteria were identified as A. baumannii. The results showed that 100% of the selected isolates were resistant to cefotaxime, ceftazidime, ciprofloxacin, piperacillin-tazobactam, and meropenem. Based on the antibiotic resistance pattern, 25 isolates were chosen for PCR analysis targeting bla_OXA-51, bla_OXA-23, bla_OXA-58, bla_NDM, bla_IMP, and bla_VIM genes PCR results revealed that among the selected isolates, 15 (60.0%) harbored the bla_OXA-23 gene, 23 (92.0%) contained the bla_OXA-51 gene, and 1 (4.0%) isolate carried the bla_NDM gene. Based on MLST analysis, two colistin-resistant Acinetobacter baumannii isolates were categorized as ST2. The ST2 clone represents the predominant sequence type within the CC2 or international clone two. The results showed that the best antibiotic against isolates was colistin. bla_OXA-51 and bla_OXA-23 genes (oxacillinase genes) were dominant genes, but bla_IMP and bla_OXA-58 were not local carbapenem resistant genes in Isfahan.

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来源期刊

BMC Research Notes Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

3.60

自引率

0.00%

发文量

363

审稿时长

15 weeks

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