Occurrence of Taenia species and Toxoplasma gondii in pigs slaughtered in Bujumbura city, Kayanza and Ngozi provinces, Burundi.

IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES
Salvator Minani, Emma Spiessens, Alyssa Labarrière, Pascal Niyokwizera, Anastasie Gasogo, Jean-Bosco Ntirandekura, Chiara Trevisan, Sarah Gabriël
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引用次数: 0

Abstract

Background: Taenia spp. and Toxoplasma gondii are foodborne parasites affecting humans and pigs. The magnitude of the burden of these parasites in pigs in Burundi is not known. Therefore, this study aimed to estimate the prevalence of Taenia spp. infections in pigs by meat inspection, partial carcass dissection and molecular confirmation and estimate the prevalence of Toxoplasma gondii infection in pigs by serology. A cross-sectional study was conducted in pig slaughter slabs located in Bujumbura city, Kayanza and Ngozi provinces. Multisampling strategies were used to sample 576 pigs. Upon pig slaughter, blood samples were collected to perform indirect ELISA for detecting antibodies against the T. gondii P30 protein in the serum. Routine meat inspection was carried out to detect T. solium and T. hydatigena cysticerci. The tongue, heart and masseter muscles were dissected by making slices less than 5 mm thick to estimate the intensity and stages of T. solium cysticerci. A subset of cysticerci and suspected lesions per infected pig were examined using PCR-RFLP to differentiate Taenia spp.

Results: Of the 576 pigs, 14 (2.4%) were positive for T. solium cysticercosis by meat inspection and 67 (11.6%) by partial carcass dissection. After molecular analysis, 66 (11.5%) samples were confirmed to be T. solium infections. The average of T. solium cysticerci in the dissected organs was estimated at 80 cysticerci. Most cysticerci (76.1%) were counted in the masseter muscles, followed by the tongue (18.8%) and the heart (5.1%). The majority of cysticerci (88.3%) were viable, 6.4% were calcified and 5.3% were degenerated. Approximately 69% of the pigs infected with T. solium had light infections, 13.4% had moderate infections and 17.9% had heavy infections. Thirty-two out of 576 pigs (5.5%) were suspected of being infected with T. hydatigena by meat inspection, but 24 pigs (4.2%) were confirmed molecularly to be positive for T. hydatigena infection. The seroprevalence of T. gondii infection in pigs was 17.7%.

Conclusions: This study indicates that T. solium and T. gondii parasites are endemic in Burundi and provides evidence of potential public health risks for the local population. Effective control strategies, including improved pig farming practices, better hygiene and sanitation, increased meat inspection, monitoring of infected pigs, risk-free culinary practices, and treatment of tapeworm carriers, should be implemented to avoid the perpetual contamination of pigs and humans with these zoonotic parasites.

布隆迪布琼布拉市、卡扬扎省和恩戈齐省屠宰的猪中出现带绦虫和刚地弓形虫。
背景:带绦虫和刚地弓形虫是影响人类和猪的食源性寄生虫。目前尚不清楚布隆迪猪中这些寄生虫负担的严重程度。因此,本研究旨在通过肉品检验、部分胴体解剖和分子鉴定等方法估计猪带绦虫感染的流行程度,并通过血清学方法估计猪刚地弓形虫感染的流行程度。在布琼布拉市、卡扬扎省和恩戈齐省的生猪屠宰场进行了横断面研究。采用多采样策略对576头猪进行采样。屠宰猪后,采集血液样本进行间接ELISA检测血清中弓形虫P30蛋白抗体。对肉品进行常规检验,检出猪螺旋体和囊虫螺旋体。用小于5毫米厚的薄片解剖舌、心和咬肌,以估计猪囊尾蚴的强度和分期。结果:576头猪中,14头(2.4%)经肉检呈猪绦虫囊虫病阳性,67头(11.6%)经部分胴体解剖呈猪绦虫囊虫病阳性。经分子分析,66份(11.5%)标本为猪弓形虫感染。在解剖器官中,猪尾绦虫囊虫的平均数量估计为80个囊虫。以咬肌囊虫最多(76.1%),其次为舌部(18.8%)和心脏(5.1%)。大多数囊虫(88.3%)有活力,钙化6.4%,变性5.3%。感染猪中约69%为轻度感染,13.4%为中度感染,17.9%为重度感染。576头猪中32头(5.5%)经肉品检验疑似猪瘟螺旋体感染,24头(4.2%)分子鉴定为猪瘟螺旋体感染阳性。猪血清弓形虫感染率为17.7%。结论:本研究表明,弓形虫和弓形虫寄生虫在布隆迪流行,并为当地人口提供了潜在公共卫生风险的证据。应实施有效的控制策略,包括改进养猪方法、改善个人卫生和环境卫生、加强肉类检查、监测受感染猪、无风险烹饪方法和治疗绦虫携带者,以避免这些人畜共患寄生虫对猪和人的永久污染。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
BMC Veterinary Research
BMC Veterinary Research VETERINARY SCIENCES-
CiteScore
4.80
自引率
3.80%
发文量
420
审稿时长
3-6 weeks
期刊介绍: BMC Veterinary Research is an open access, peer-reviewed journal that considers articles on all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.
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