{"title":"A Mitochondria-Targeted Iridium(iii) Phosphorescent Probe for Selective Detection of Hypochlorite in Living Cells.","authors":"Wenjuan Xu, Haoxuan Wang, Ying Guang, Zhiqiang Pan, Kai Chen, Tingchun Ma, Jing Zhang","doi":"10.1002/asia.202401351","DOIUrl":null,"url":null,"abstract":"<p><p>Hypochlorous acid(HClO)/hypochlorite ion (ClO-) is a highly reactive oxygen species (ROS) that play a crucial role in various biological processes. In this paper, a \"turn-on\" phosphorescent probe (Ir-TPP) for detecting ClO- in mitochondria was designed and synthesized. In solution, Ir-TPP is minimal emission due to rapid isomerization of C=N-OH as an efficient non-radiative decay process. When Ir-TPP was reacted with ClO- to form an oxidized carboxylate product, there is a significant enhancement both in absorbance and emission intensity, resulting in approximately 19-fold increase in phosphorescence signal. Furthermore, the probe demonstrates high selectivity towards other anions and ROS. It also exhibits specific targeting ability towards mitochondria with a co-staining coefficient of 94% compared to commercial dyes, enabling \"turn-on\" detection of ClO- within mitochondria.</p>","PeriodicalId":145,"journal":{"name":"Chemistry - An Asian Journal","volume":" ","pages":"e202401351"},"PeriodicalIF":3.5000,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemistry - An Asian Journal","FirstCategoryId":"1","ListUrlMain":"https://doi.org/10.1002/asia.202401351","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Hypochlorous acid(HClO)/hypochlorite ion (ClO-) is a highly reactive oxygen species (ROS) that play a crucial role in various biological processes. In this paper, a "turn-on" phosphorescent probe (Ir-TPP) for detecting ClO- in mitochondria was designed and synthesized. In solution, Ir-TPP is minimal emission due to rapid isomerization of C=N-OH as an efficient non-radiative decay process. When Ir-TPP was reacted with ClO- to form an oxidized carboxylate product, there is a significant enhancement both in absorbance and emission intensity, resulting in approximately 19-fold increase in phosphorescence signal. Furthermore, the probe demonstrates high selectivity towards other anions and ROS. It also exhibits specific targeting ability towards mitochondria with a co-staining coefficient of 94% compared to commercial dyes, enabling "turn-on" detection of ClO- within mitochondria.
期刊介绍：
Chemistry—An Asian Journal is an international high-impact journal for chemistry in its broadest sense. The journal covers all aspects of chemistry from biochemistry through organic and inorganic chemistry to physical chemistry, including interdisciplinary topics.
Chemistry—An Asian Journal publishes Full Papers, Communications, and Focus Reviews.
A professional editorial team headed by Dr. Theresa Kueckmann and an Editorial Board (headed by Professor Susumu Kitagawa) ensure the highest quality of the peer-review process, the contents and the production of the journal.
Chemistry—An Asian Journal is published on behalf of the Asian Chemical Editorial Society (ACES), an association of numerous Asian chemical societies, and supported by the Gesellschaft Deutscher Chemiker (GDCh, German Chemical Society), ChemPubSoc Europe, and the Federation of Asian Chemical Societies (FACS).
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