A novel approach to detect CD4 T-lymphocytes using a microfluidic chip and compact signal processing circuit

IF 2.3 4区工程技术 Q2 INSTRUMENTS & INSTRUMENTATION

Microfluidics and Nanofluidics Pub Date : 2024-12-31 DOI:10.1007/s10404-024-02782-4

Huong Thi Phi, Phu Van Nguyen, Thanh Van Pham, Huy Van Hoang, Quynh Manh Luu, Thien Duy Nguyen, Huong Thi Thu Pham, Van Thi Thanh Nguyen, Luong Hoang Nguyen, Hong Thi Tran, Nam Hoang Nguyen

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引用次数: 0

Abstract

CD4 T-lymphocytes (CD4 cells) are a type of T lymphocyte that plays an important role in the immune system, helping to fight germs and protect the body from disease. Accurate enumeration of CD4 T cells is crucial for assessing immune health and diagnosing various diseases. This study presents the development and validation of a novel microfluidic biochip system designed for the detection and counting of CD4 T cells using impedance measurements. The proposed system integrated a cell detection chip with a cost-effective signal processing circuit, which included an instrumental amplifier and a highly sensitive lock-in amplifier. The sensing structure, created using advanced microfabrication technology, consists of three microelectrodes and a 50 × 50 μm measurement aperture. The detection principle relied on the impedance imbalance caused by the presence of CD4 T cells in the fluidic flow between adjacent sensing electrodes. The system's performance was validated through extensive experiments, demonstrating high accuracy in detecting and counting CD4 T cells separated from whole blood based on their magnetic properties. The experimental results indicate that the proposed system was simpler, faster, and more cost-effective compared to traditional laser flow cytometry. Furthermore, the system’s portability and ease of use made it highly suitable for point-of-care diagnostics and on-site cell analysis. The utilization of microfabrication technology and impedance measurement not only enhanced efficiency and accuracy but also offered a reliable solution for rapid biological cell detection. Future work will focus on enhancing the throughput and miniaturizing the sensing structure to align with the high standards of conventional flow cytometry while maintaining cost-effectiveness and simplicity. This research lays a solid foundation for the development of advanced lab-on-a-chip technologies for biological cell detection and analysis, promising significant improvements in healthcare diagnostics and monitoring.

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一种利用微流控芯片和紧凑信号处理电路检测CD4 t淋巴细胞的新方法

CD4 T淋巴细胞（CD4细胞）是T淋巴细胞的一种，在免疫系统中起着重要作用，帮助对抗细菌和保护身体免受疾病的侵害。CD4 T细胞的准确计数对于评估免疫健康和诊断各种疾病至关重要。本研究提出了一种新型微流控生物芯片系统的开发和验证，该系统设计用于使用阻抗测量检测和计数CD4 T细胞。该系统集成了一个细胞检测芯片和一个具有成本效益的信号处理电路，其中包括一个仪器放大器和一个高灵敏度的锁定放大器。该传感结构采用先进的微加工技术，由三个微电极和一个50 × 50 μm的测量孔径组成。检测原理依赖于相邻传感电极之间的流体流动中CD4 T细胞的存在所引起的阻抗不平衡。通过大量的实验验证了该系统的性能，证明了基于其磁性来检测和计数从全血中分离的CD4 T细胞的高精度。实验结果表明，与传统的激光流式细胞术相比，该系统更简单、快速、经济。此外，该系统的便携性和易用性使其非常适合于即时诊断和现场细胞分析。微加工技术和阻抗测量的应用不仅提高了效率和精度，而且为生物细胞的快速检测提供了可靠的解决方案。未来的工作将集中在提高通量和小型化传感结构，以符合传统流式细胞术的高标准，同时保持成本效益和简单性。这项研究为生物细胞检测和分析的先进芯片实验室技术的发展奠定了坚实的基础，有望在医疗保健诊断和监测方面取得重大进展。

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来源期刊

Microfluidics and Nanofluidics 工程技术-纳米科技

CiteScore

4.80

自引率

3.60%

发文量

审稿时长

2 months

期刊介绍： Microfluidics and Nanofluidics is an international peer-reviewed journal that aims to publish papers in all aspects of microfluidics, nanofluidics and lab-on-a-chip science and technology. The objectives of the journal are to (1) provide an overview of the current state of the research and development in microfluidics, nanofluidics and lab-on-a-chip devices, (2) improve the fundamental understanding of microfluidic and nanofluidic phenomena, and (3) discuss applications of microfluidics, nanofluidics and lab-on-a-chip devices. Topics covered in this journal include: 1.000 Fundamental principles of micro- and nanoscale phenomena like, flow, mass transport and reactions 3.000 Theoretical models and numerical simulation with experimental and/or analytical proof 4.000 Novel measurement & characterization technologies 5.000 Devices (actuators and sensors) 6.000 New unit-operations for dedicated microfluidic platforms 7.000 Lab-on-a-Chip applications 8.000 Microfabrication technologies and materials Please note, Microfluidics and Nanofluidics does not publish manuscripts studying pure microscale heat transfer since there are many journals that cover this field of research (Journal of Heat Transfer, Journal of Heat and Mass Transfer, Journal of Heat and Fluid Flow, etc.).