The significance of MDK growth factor in the antler development of sika deer (Cervus nippon): An in-depth analysis.

Abstract

Deer antlers exhibit rapid growth during the velvet phase. As a critical endogenous growth factor in animals, midkine (MDK) is likely closely associated with the growth of antlers. However, the spatio-temporal expression pattern of MDK during the velvet phase was unclear. This study explored the physiological role of MDK by analyzing its molecular characterization and spatio-temporal expression dynamics during the growth of sika deer antlers. The study cloned the coding sequences (CDS) of MDK, which spanned 429 bp and encoded 142 amino acids. The results of bioinformatics prediction analysis showed that MDK was an extracellular hydrophilic secreted protein, which was mainly composed of random coil. MDK protein was relatively conserved in evolution and MDK protein of sika deer had the closest relatives to ruminants and the furthest relatives to Aves. The tip tissues (dermis, mesenchyme, precartilage, cartilage) of antlers were collected from three important growth and development nodes (early period, EP. middle period, MP. late period, LP), and quantitative real-time polymerase chain reaction (qRT-PCR) was chosen to detect the spatio-temporal expression of the MDK. The results showed that MDK was expressed in all tissue sites of antler tip in EP, MP, LP. MDK had a consistent expression pattern under all growth periods and was strongly expressed in dermis and cartilage. The expression of MDK was consistently up-regulated in precartilage, whereas it was first up-regulated and then down-regulated in other tissues, and it was highly significant in MP compared to EP and LP (P < 0.01). This study suggested that MDK may regulate the growth of dermis and cartilage tissues mainly by participating in the process of angiogenesis and bone formation, thus promoting the rapid growth of antlers.