Berbamine inhibits Pseudorabies virus in vitro and in vivo

IF 2.4 2区农林科学 Q3 MICROBIOLOGY

Veterinary microbiology Pub Date : 2025-02-01 DOI:10.1016/j.vetmic.2024.110356

Liang Li , Muze Lv , Yangfan Li , Huihui Sun , Jie Li , Wenyan Li , Xuan Wang , Ruimin Bi , Zuyao Zhang , Zongyi Bo , Haixiao Shen , Jun Wang , Minghao Zhuansun , Jinchi Zhou , Yuting Xue , Xinru Suo , Rui Tong , Pei Sun

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引用次数: 0

Abstract

Pseudorabies virus (PRV) is a significant pathogen that causes acute infectious diseases in pigs, resulting in considerable economic losses for the global pig industry. The lack of effective control measures and vaccines against the circulating variants of PRV highlights the pressing need for novel treatment strategies. In this study, a screening of a natural product library identified Berbamine as a promising compound that inhibits PRV replication, with a selectivity index of 17. Preliminary investigations demonstrated that Berbamine impedes viral proliferation by targeting the replication and release stages of the PRV life cycle. In experiments with mice artificially infected with PRV, Berbamine significantly alleviated clinical symptoms and histopathological changes in brain tissue caused by PRV infection. Furthermore, molecular docking studies indicated that Berbamine targets the UL50 protein, not only of PRV but also of HSV-1, FHV-1, and BoHV-1. Given that the UL50 protein is a promising target for antiviral drug development, Berbamine holds considerable potential for broad application in antiviral therapies.

查看原文本刊更多论文

小檗碱在体外和体内抑制伪狂犬病毒。

伪狂犬病毒（PRV）是引起猪急性传染病的重要病原体，给全球养猪业造成相当大的经济损失。由于缺乏有效的控制措施和疫苗来对抗流行的PRV变种，因此迫切需要新的治疗策略。本研究通过对天然产物文库的筛选，发现Berbamine是一种抑制PRV复制的有前景的化合物，其选择性指数为17。初步研究表明，Berbamine通过靶向PRV生命周期的复制和释放阶段来阻碍病毒增殖。在人工感染PRV的小鼠实验中，Berbamine显著缓解了PRV感染引起的临床症状和脑组织组织病理改变。此外，分子对接研究表明，Berbamine不仅靶向PRV的UL50蛋白，还靶向HSV-1、FHV-1和BoHV-1的UL50蛋白。鉴于UL50蛋白是抗病毒药物开发的一个有希望的靶点，Berbamine在抗病毒治疗中具有相当大的广泛应用潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary microbiology 农林科学-兽医学

CiteScore

5.90

自引率

6.10%

发文量

221

审稿时长

52 days

期刊介绍： Veterinary Microbiology is concerned with microbial (bacterial, fungal, viral) diseases of domesticated vertebrate animals (livestock, companion animals, fur-bearing animals, game, poultry, fish) that supply food, other useful products or companionship. In addition, Microbial diseases of wild animals living in captivity, or as members of the feral fauna will also be considered if the infections are of interest because of their interrelation with humans (zoonoses) and/or domestic animals. Studies of antimicrobial resistance are also included, provided that the results represent a substantial advance in knowledge. Authors are strongly encouraged to read - prior to submission - the Editorials (''Scope or cope'' and ''Scope or cope II'') published previously in the journal. The Editors reserve the right to suggest submission to another journal for those papers which they feel would be more appropriate for consideration by that journal. Original research papers of high quality and novelty on aspects of control, host response, molecular biology, pathogenesis, prevention, and treatment of microbial diseases of animals are published. Papers dealing primarily with immunology, epidemiology, molecular biology and antiviral or microbial agents will only be considered if they demonstrate a clear impact on a disease. Papers focusing solely on diagnostic techniques (such as another PCR protocol or ELISA) will not be published - focus should be on a microorganism and not on a particular technique. Papers only reporting microbial sequences, transcriptomics data, or proteomics data will not be considered unless the results represent a substantial advance in knowledge. Drug trial papers will be considered if they have general application or significance. Papers on the identification of microorganisms will also be considered, but detailed taxonomic studies do not fall within the scope of the journal. Case reports will not be published, unless they have general application or contain novel aspects. Papers of geographically limited interest, which repeat what had been established elsewhere will not be considered. The readership of the journal is global.