Application of an Innovative Embedding and Cooling Component in the Critical Steps of Fragmented Tissue Pathological Diagnosis.

Abstract

This study aimed to explore the application value of an innovative embedding and cooling component in the critical steps of fragmented tissue pathological diagnosis. Eighty small and fragmented tissue samples were collected and randomly divided into two groups. The embedding qualification rate and embedding efficiency were compared between the two groups. Seventy cases of fragmented tissue were collected, with three pieces of fragmented tissue taken from each case. The samples were randomly divided into three groups for sectioning. The wrinkle-free scores and sectioning efficiency were compared between different cooling methods. The embedding qualification rate was 90.0% in the embedding tweezer group, which was lower than 98.8% in the innovative component group (χ² = 4.24, p < 0.05). The average embedding time per sample was 25.08 ± 4.61 s in the embedding tweezer group, which was significantly longer than the 16.69 ± 3.54 s in the innovative component group (t = 12.91, p < 0.001). The wrinkle-free section scores were 8.24 ± 0.69 in the small ice block cooling group, 8.11 ± 0.74 in the freezing platform cooling group, and 8.85 ± 0.65 in the innovative component cooling group, with the innovative component cooling group scoring significantly higher than the other two groups (q = 7.40, 8.98, p < 0.01). The innovative component cooling group had a shorter average sectioning time than the other two groups (q = 3.12, 26.75, p < 0.05, 0.01). The use of the innovative embedding and cooling component optimizes the embedding and sectioning steps in the pathological diagnosis of fragmented tissue. This method is worthy of promotion and application.