The Efficacy of Liquid Biopsy of Total cfDNA for Predicting Systemic Metastasis in Japanese Patients With Oral Squamous Cell Carcinoma.

IF 2.3 3区医学 Q1 OTORHINOLARYNGOLOGY

Head and Neck-Journal for the Sciences and Specialties of the Head and Neck Pub Date : 2024-12-27 DOI:10.1002/hed.28054

Masahiro Uchibori, Kazuyoshi Hosomichi, Yasutaka Hoshimoto, Masashi Sasaki, Takayuki Aoki, Atsushi Tajima, Yoshihide Ota, Minoru Kimura

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引用次数: 0

Abstract

Background: The use of liquid biopsy of total cell-free DNA (cfDNA) to identify otherwise undetectable cancers has attracted interest; however, its efficacy remains unknown. We explored whether analysis using total cfDNA is efficacious for Japanese patients with oral squamous cell carcinoma (OSCC).

Methods: We collected total cfDNA from nine patients with OSCC preoperatively, 1 month postoperatively, and every 3 months thereafter to analyze this association. We used a target DNA sequence for genetic mutation analysis of tumor tissues collected from 33 patients, including the aforementioned nine patients.

Results: Patients with good disease control showed negligible changes in preoperative and postoperative total cfDNA concentrations. A rapid increase in total cfDNA concentration was observed in patients who developed systemic metastases. Patients whose tumor tissue DNA showed genetic mutations had the same mutations in preoperative circulating tumor DNA.

Conclusions: Our data suggested that analyzing total cfDNA is efficacious for patients with OSCC.

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总cfDNA液体活检预测日本口腔鳞状细胞癌系统性转移的疗效。

背景：使用液体活检的总游离细胞DNA （cfDNA）来识别其他无法检测的癌症已经引起了人们的兴趣；然而，其功效尚不清楚。我们探讨了总cfDNA分析对日本口腔鳞状细胞癌（OSCC）患者是否有效。方法：收集9例OSCC患者术前、术后1个月及术后每3个月的cfDNA，分析其相关性。我们使用目标DNA序列对33例患者的肿瘤组织进行基因突变分析，其中包括上述9例患者。结果：疾病控制良好的患者术前和术后总cfDNA浓度变化可忽略不计。在发生全身转移的患者中观察到总cfDNA浓度迅速增加。肿瘤组织DNA发生基因突变的患者术前循环肿瘤DNA发生相同的突变。结论：我们的数据表明分析总cfDNA对OSCC患者是有效的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Head and Neck-Journal for the Sciences and Specialties of the Head and Neck 医学-耳鼻喉科学

CiteScore

7.00

自引率

6.90%

发文量

278

审稿时长

1.6 months

期刊介绍： Head & Neck is an international multidisciplinary publication of original contributions concerning the diagnosis and management of diseases of the head and neck. This area involves the overlapping interests and expertise of several surgical and medical specialties, including general surgery, neurosurgery, otolaryngology, plastic surgery, oral surgery, dermatology, ophthalmology, pathology, radiotherapy, medical oncology, and the corresponding basic sciences.