Photosensitizing Activity of Nanoparticles of Poly (2-amino phenol)/Gold for Intensified Doxorubicin Therapeutic Effect on Melanoma Cancer Cells under Synergism Effect of 808-nm Light.

Q3 Medicine

Journal of Biomedical Physics and Engineering Pub Date : 2024-12-01 DOI:10.31661/jbpe.v0i0.2312-1693

Naghmeh Sattarahmady, Zahra Kayani, Hossein Heli, Parsa Faghani-Eskandarkolaei, Hanieh Haghighi

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引用次数: 0

Abstract

Background: Photothermal therapy (PTT) is one of the effective and non-invasive strategies which hold great promise for improving the treatment of cancer cells. PTT is based on activating a photosensitizer by infrared light irradiation and producing heat and reactive species and apoptosis in the tumor area.

Objective: The aim of this study was to investigate the effect of photothermal/chemotherapy on melanoma cancer cells using poly (2-amino phenol)/gold (P2AO/AuNPs) and doxorubicin (DOX).

Material and methods: In this experimental study, nanoparticles of P2AO/AuNPs were synthesized, and their mixture with DOX was applied as a photosensitizer for photothermal/chemotherapy of a C540 (B16-F10) melanoma cell line.

Results: P2AO/AuNPs generated heat and cytotoxic responsive oxygen species (ROS) upon 808-nm light irradiation with simultaneous intensifying DOX therapeutic effect under domination of synergism effects between light irradiation, P2AO/AuNPs, and doxorubicin. Cell treatment with both P2AO/AuNPs and DOX resulted in a considerable increase in necroptotic cells to 61% with a significant decrease in the living cells (39%).

Conclusion: P2AO/AuNPs provided a platform for light absorption and intensifying DOX therapeutic effect. This study approved the applicability of a new photothermal/chemotherapy by domination of synergistic effects attained by combination of laser light, P2AO, AuNPs, and DOX.

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808 nm光协同作用下聚（2-氨基酚）/金纳米颗粒增强阿霉素治疗黑色素瘤癌细胞的光敏活性

背景：光热疗法（PTT）是一种有效的、非侵入性的治疗方法，有望改善肿瘤细胞的治疗。PTT的基础是通过红外光照射激活光敏剂，在肿瘤区域产生热量和活性物质和细胞凋亡。目的：探讨聚（2-氨基酚）/金（P2AO/AuNPs）和多柔比星（DOX）光热/化疗对黑色素瘤癌细胞的影响。材料与方法：本实验研究合成了P2AO/AuNPs纳米颗粒，并将其与DOX混合作为光敏剂应用于C540 （B16-F10）黑色素瘤细胞系的光热/化疗。结果：在808 nm光照射下，P2AO/AuNPs在光照射、P2AO/AuNPs和阿霉素的协同作用下，产生热量和细胞毒性反应氧（ROS），同时增强DOX的治疗效果。用P2AO/AuNPs和DOX处理细胞导致坏死细胞显著增加至61%，而活细胞显著减少（39%）。结论：P2AO/AuNPs为光吸收提供平台，增强DOX的治疗效果。这项研究通过激光、P2AO、AuNPs和DOX联合获得的协同效应，证实了一种新的光热/化疗的适用性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Biomedical Physics and Engineering Medicine-Radiology, Nuclear Medicine and Imaging

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

10 weeks

期刊介绍： The Journal of Biomedical Physics and Engineering (JBPE) is a bimonthly peer-reviewed English-language journal that publishes high-quality basic sciences and clinical research (experimental or theoretical) broadly concerned with the relationship of physics to medicine and engineering.