Protective effect of Ocimum basilicum L. essential oil on Lactuca sativa L. treated with cadmium.

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2024-12-21 DOI:10.1016/j.plaphy.2024.109437

Simone Landi, Flavio Polito, Sergio Esposito, Sergio Sorbo, Piergiorgio Cianciullo, Alessia Postiglione, Vincenzo De Feo, Adriana Basile, Viviana Maresca

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Abstract

In recent years, essential oils (EO) are a sustainable and effective alternative to conventional chemical treatments in response to heavy metals in plants. These natural molecules can increase the resilience of plants under stress conditions. In the present work, the ability of EOs from the aerial parts of Ocimum basilicum L. cv 'Prospera' to improve plant response to heavy metals in Lactuca sativa L. grown hydroponically and subjected to Cd stress was investigated. The chemical profile of the essential oil (EO) was analyzed by GC-MS. Essential oil-induced tolerance to different Cd concentrations (36 μM and 72 μM) was studied by analyzing ultrastructural damage by TEM observations, antioxidant response by spectrophotometric analysis, and changes in gene expression by qRT-PCR involved in abiotic stress response. Our results indicated that exogenous EO application of basil helps preserve plastid ultrastructure and ameliorates Cd-induced damage. In addition, there was a reduction in ROS production and beneficial regulation of the activities and molecular expression of antioxidant enzymes. In conclusion, these results clearly indicate the protective ability of basil EO on cytological organization and in modulating the redox state through the antioxidant pathway, reducing Cd-induced oxidative stress.

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罗勒精油对镉胁迫下芥蓝的保护作用。

近年来，植物精油是一种可持续有效的化学处理方法，可以替代传统的化学处理方法来处理植物中的重金属。这些天然分子可以增加植物在逆境条件下的恢复能力。本研究以水栽和Cd胁迫下的芥蓝（lacuca sativa L.）为研究材料，研究了芫花（Ocimum basilicum L. cv 'Prospera'）地上部分的EOs提高植株对重金属响应的能力。采用气相色谱-质谱法分析了精油的化学成分。通过透射电镜观察精油对不同Cd浓度（36 μM和72 μM）的超微结构损伤，分光光度分析抗氧化反应，qRT-PCR研究精油对不同Cd浓度（36 μM和72 μM）的耐受性。结果表明，外源EO处理有助于保持罗勒质体超微结构，改善cd诱导的损伤。此外，活性氧的产生减少，抗氧化酶的活性和分子表达也受到有益的调节。综上所述，这些结果清楚地表明罗勒EO对细胞组织具有保护作用，并通过抗氧化途径调节氧化还原状态，减轻cd诱导的氧化应激。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.