Physiological and transcriptomic analysis of purple flowering stalks (Brassica campestris var. purpurea) under cadmium stress and exogenous glutathione application.

Abstract

Glutathione (GSH) has a beneficial effect on the response of plants to cadmium (Cd) stress. The physiological and molecular processes by which glutathione influences Cd tolerance in purple flowering stalks (a Brassica vegetable) remain unclear. The aim of this study was to investigate the role of exogenous GSH in alleviating Cd toxicity in purple flowering stalks. On day 10 of the Cd stress treatment, spraying of GSH resulted in an increase in the net photosynthetic rate by 18.48%; enhanced antioxidant enzyme activities and the endogenous GSH and ascorbic acid contents; reduced the malondialdehyde and proline content 32.45% and 24.65%, respectively; and reduced the Cd content in the roots by 2.93%. On day 5, the transcriptome analysis showed that the application of GSH up-regulated the expression of 27 genes in the photosynthetic pathway. In contrast, GSH application led to the down-regulation of most genes involved in GSH metabolism, sulfur metabolism, and arginine and proline metabolism. These findings will aid future studies of the response of purple flowering stalks to Cd stress.