High-throughput identification of Prunus mume freezing-tolerance genes based on yeast screening system and functional verification of PmRCI2-3 in Arabidopsis.

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2024-12-20 DOI:10.1016/j.plaphy.2024.109431

Lichen Yang, Xue Li, Ke Chen, Zhiyuan Ma, Like Qiu, Chongyang Yuan, Jia Wang, Tangren Cheng, Qixiang Zhang, Tangchun Zheng

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Abstract

Prunus mume tops the ten most famous flowers of China with high ornamental value, and low temperature is the main factor limiting its northward migration. Cold resistance improvement is one of the important breeding directions of Rosaceae ornamental plants, especially the Prunus mume. Here, 29 genes from P. mume were screened by yeast screening system under -20 °C for 96 h. Based on GO and KEGG analysis, rare cold-inducible 2 family gene member PmRCI2-3 was first cloned for functional verification. Subcellular localization results showed the PmRCI2-3 was located in the membrane structure, and GUS staining showed that the activity of the PmRCI2-3 promoter was spatiotemporally specific. Overexpression PmRCI2-3 in Arabidopsis thaliana can reduce plant damage at low temperatures. The expression levels of endogenous genes (AtCBF1, AtCBF2, AtCBF3, AtCOR15A, and AtRD29A) related to cold response were all up-regulated, except AtKIN was down-regulated. These results lay the foundation for further providing key candidate genes for cold resistance breeding of P. mume and other Prunus species.

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基于酵母筛选系统的拟南芥李抗冻基因高通量鉴定及PmRCI2-3功能验证

梅李是中国十大名花之一，具有很高的观赏价值，低温是制约其北迁的主要因素。抗寒性改良是蔷薇科观赏植物，尤其是梅李的重要育种方向之一。本研究利用酵母筛选系统在-20°C条件下对29个基因进行筛选，筛选时间为96 h。基于GO和KEGG分析，首次克隆出罕见的冷诱导2家族基因成员PmRCI2-3进行功能验证。亚细胞定位结果显示PmRCI2-3位于膜结构中，GUS染色显示PmRCI2-3启动子的活性具有时空特异性。PmRCI2-3在拟南芥中过表达可以减少低温对植株的伤害。除AtKIN下调外，与冷反应相关的内源基因AtCBF1、AtCBF2、AtCBF3、AtCOR15A、AtRD29A的表达水平均上调。这些结果为进一步为梅梅等李属植物的抗寒育种提供关键候选基因奠定了基础。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.