Evaluation of antibiotic purchase data for ceftiofur and enrofloxacin and minimum inhibitory concentrations among Escherichia coli isolates from swine farms in the Midwestern United States using multiple statistical models.

Abstract

Antimicrobial resistance is considered a global One Health threat. Controlling selection pressure by reducing antibiotic use in livestock is a significant component of the response to this threat. The science concerning use and resistance is complicated and affected by time from antibiotic exposure, changing bacterial fitness, and varies by drug and pathogen. From May 2020 through October 2023, we collected intestinal (substandard and sick pigs) and fecal swab (healthy pig) samples at breed-to-wean (BTW) and wean-to-market (WTM) swine production sites and isolated E. coli bacteria. Antibiotic susceptibility testing was performed on these isolates to determine minimum inhibitory concentrations (MIC) for ceftiofur and enrofloxacin. Monthly antibiotic purchase data were used to calculate the active milligrams of drug purchased and these were divided by the kilograms of pigs produced from a farm site to provide a mass-adjusted proxy metric for farm-level antibiotic use. The relationship between use and MIC was then evaluated using a variety of multivariable statistical models. Across multiple modeling approaches, both farm type (i.e., BTW versus WTM) and farm-level antibiotic use maintained statistically significant relationships relative to E. coli MIC values for each respective drug. Use of ceftiofur and enrofloxacin can lead to increased MIC values among E. coli over time. The reasons for antibiotic purchases were not tracked as part of this project. Future work should evaluate the age of the individual pig and the time from last treatment when sampling these animals to separate out the group from individual-level effects of antibiotic use.