Genetic profiling of Plasmodium falciparum antigenic biomarkers among asymptomatic pregnant women on intermittent preventive treatment with sulfadoxine-pyrimethamine from southwest Nigeria

IF 3 2区医学 Q2 DEVELOPMENTAL BIOLOGY

Placenta Pub Date : 2025-01-01 DOI:10.1016/j.placenta.2024.12.016

R.I. Funwei , A. Olaleye , G.N. Uyaiabasi , W. Hammed , M.M. Obadimeji , C.J. Elikwu , A. Adepoju , C. Okangba , A. Akinyede , O. Ojurongbe , C. Falade , O. Walker

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引用次数: 0

Abstract

Introduction

The genetic complexity of Plasmodium falciparum is contributory to the emergence of drug resistant-parasites. Intermittent preventive treatment of malaria in pregnancy with sulfadoxine-pyrimethamine (IPTp-SP) in malaria endemic settings is recommended by WHO. This study evaluated the prevalence of Plasmodium falciparum multidrug resistance-1 gene (Pfmdr-1), genetic diversity of merozoite surface proteins (msp-1, msp-2) and glutamate-rich protein (glurp) among pregnant women with sub-patent parasitaemia from southwest Nigeria.

Methods

One hundred PCR-confirmed Plasmodium falciparum isolates, collected at first visit-V-1 (n = 52), delivery (n = 31) and cord blood (n = 17), were selected for analysis. The Pfmdr-1 alleles was evaluated using restriction fragment length polymorphism (RLFP), while msp-1, msp-2 and glurp genes were genotyped. Allelic frequency distribution and multiplicity of infection were calculated at p-value ≤0.05.

Results

The Pfmdr-1 (N86/N86Y) combination was detected in 11.8 %, 61.3 % and 58.8 % (p ≤ 0.05) in V-1, Delivery and Cord isolates respectively. The N86Y haplotype was detected only in cord (5.9 %). The allelic frequency distribution for msp-1 was 244 (K1 = 81, MAD20 = 84 and RO33 = 79), and msp-2; 110 alleles, representing 43.6 % (FC27) and 56.4 % (3D7). While glurp expressed 25 alleles, 84 % (V-1), 12 % (delivery) and 4 % (cord), respectively (p ≤ 0.05). The msp-1 and msp-2 recorded higher MOIs than glurp.

Discussion

Genetically diverse P. falciparum strains with Pfmdr-1 mutant alleles were detected in pregnant women with sub-patent parasitaemia in southwest Nigeria, which may reduce IPTp-SP effectiveness. Thus, continuous molecular surveillance of resistant-parasites to sulphadoxine-pyrimethamine and ACTs is essential.

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尼日利亚西南部接受磺胺多辛-乙胺嘧啶间歇预防治疗的无症状孕妇中恶性疟原虫抗原生物标志物的遗传分析

恶性疟原虫的遗传复杂性有助于耐药寄生虫的出现。世卫组织建议在疟疾流行环境中用磺胺多辛-乙胺嘧啶（IPTp-SP）间歇预防性治疗妊娠期疟疾。本研究评估了尼日利亚西南部亚潜伏性寄生虫病孕妇恶性疟原虫多药耐药1基因（Pfmdr-1）的流行情况、胚子表面蛋白（msp-1、msp-2）和谷氨酸丰富蛋白（glurp）的遗传多样性。方法：选取首次访诊时采集的经pcr证实的恶性疟原虫分离株（v -1型52株，分娩31株，脐带血17株）进行分析。采用限制性内切片段长度多态性（RLFP）对Pfmdr-1等位基因进行鉴定，对msp-1、msp-2和glurp基因进行分型。计算等位基因频率分布和感染次数，p值≤0.05。结果：在V-1株、Delivery株和Cord株中，Pfmdr-1 （N86/N86Y）联合检出率分别为11.8%、61.3%和58.8% （p≤0.05）。N86Y单倍型仅在脐带中检测到（5.9%）。msp-1等位基因频率分布为244 (K1 = 81, MAD20 = 84, RO33 = 79), msp-2；110个等位基因，分别占43.6% （FC27）和56.4% （3D7）。glurp表达25个等位基因，分别为84% （V-1）、12%（分娩）和4%（脐带）（p≤0.05）。msp-1和msp-2的moi值高于glurp。讨论：在尼日利亚西南部亚专利寄生虫病孕妇中检测到具有Pfmdr-1突变等位基因的遗传多样性恶性疟原虫菌株，这可能会降低IPTp-SP的有效性。因此，对磺胺嘧啶-乙胺嘧啶和以青蒿素为基础的青蒿素类药物具有耐药性的寄生虫进行持续的分子监测至关重要。

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来源期刊

Placenta 医学-发育生物学

CiteScore

6.30

自引率

10.50%

发文量

391

审稿时长

78 days

期刊介绍： Placenta publishes high-quality original articles and invited topical reviews on all aspects of human and animal placentation, and the interactions between the mother, the placenta and fetal development. Topics covered include evolution, development, genetics and epigenetics, stem cells, metabolism, transport, immunology, pathology, pharmacology, cell and molecular biology, and developmental programming. The Editors welcome studies on implantation and the endometrium, comparative placentation, the uterine and umbilical circulations, the relationship between fetal and placental development, clinical aspects of altered placental development or function, the placental membranes, the influence of paternal factors on placental development or function, and the assessment of biomarkers of placental disorders.