Isolation and Characterization of Bioactive Compounds from Saccharomonospora sp. CMS18 and Their Antifungal Properties.

IF 4.9 2区医学 Q1 CHEMISTRY, MEDICINAL

Marine Drugs Pub Date : 2024-11-30 DOI:10.3390/md22120539

Soohyun Um, Hyeongju Jeong, Ji-Eun Park, Jeongwon Seo, Sang Heon Jung, Munhyung Bae, Kyung-Tae Lee, Kyuho Moon

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Abstract

In this study, metagenomic analysis was employed to investigate the bacterial communities in the Muan tidal mudflat of the Republic of Korea. We used metagenomic analysis to identify the microbial community in tidal soil dominated by Proteobacteria. From this environment, the bacterial strain, Saccharomonospora sp. CMS18, was isolated and yielded two previously unknown compounds, penipaline D (3) and N-acetyl-dimethylallyltryptophan (4). The chemical structures of the isolated compounds along with 6-dimethylallyl-indole (1), 6-dimethylallyltryptophan (2), penipaline D (3), and N-acetyl-dimethylallyltryptophan (4) were structurally investigated using HR-ESI-MS and NMR spectroscopy. The isolated compound 6-dimethylallyl-indole (1) demonstrated broad-spectrum antifungal activity, with IC₅₀ value of 0.04 mM against Candida glabrata and 0.35 mM against both Candida albicans and Cryptococcus neoformans. Additionally, it exhibited additive interaction with caspofungin against C. albicans.

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Saccharomonospora sp. CMS18生物活性物质的分离、鉴定及抑菌活性研究

本研究采用宏基因组分析方法对韩国务安潮滩的细菌群落进行了研究。我们利用宏基因组分析鉴定了潮土中以变形菌门（Proteobacteria）为主的微生物群落。从该环境中分离出一株Saccharomonospora sp. CMS18，分离得到两种未知化合物：penipaline D(3)和N-acetyl-dimethylallyltryptophan(4)。利用ir - esi - ms和NMR对分离得到的化合物与6-dimethylallyl-吲哚(1)、6-dimethylallyltryptophan(2)、penipaline D(3)和N-acetyl-dimethylallyltryptophan(4)进行了结构分析。分离得到的化合物6-二甲基烯丙基吲哚(1)具有广谱抗真菌活性，对光念珠菌的IC50值为0.04 mM，对白色念珠菌和新生隐球菌的IC50值为0.35 mM。此外，它与caspofunin对白色念珠菌表现出加性相互作用。

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来源期刊

Marine Drugs 医学-医药化学

CiteScore

9.60

自引率

14.80%

发文量

671

审稿时长

1 months

期刊介绍： Marine Drugs (ISSN 1660-3397) publishes reviews, regular research papers and short notes on the research, development and production of drugs from the sea. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible, particularly synthetic procedures and characterization information for bioactive compounds. There is no restriction on the length of the experimental section.