Characterization of the probiotic and functional properties of Enterococcus faecalis AQ10 isolated from chicken cecum.

IF 2 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Letters in Applied Microbiology Pub Date : 2024-12-02 DOI:10.1093/lambio/ovae116

Haichang Yin, Xinyu Zhang, Xinjie Jiang, Di Liu

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引用次数: 0

Abstract

Lactic acid bacteria are considered the safest alternative to antibiotics and have a broad range of applications in animal husbandry. Enterococcus faecalis is a primary component of the chicken gut microbiota; it is known for its ability to regulate intestinal microbial balance and its probiotic functions. In this study, E. faecalis strain AQ10 from isolated from was the chicken cecum and used a combination of whole-genome sequencing and phenotypic analyses to investigate its probiotic properties. Whole-genome sequencing revealed that the genome length of E. faecalis AQ10 is 2.98 Mbp, with an average guanine-cytosine content of 38.12%, and includes 2832 protein-coding genes. Genome mining tools were used to identify an antimicrobial compound gene cluster. Additionally, E. faecalis AQ10 exhibited probiotic characteristics in vivo and antibacterial effects in vitro, with organic acids potentially contributing to its anti-Klebsiella pneumoniae activity. Metabolomics analyses revealed that the supernatant of E. faecalis AQ10 contained seven organic acids. In conclusion, E. faecalis AQ10 demonstrates significant probiotic potential and may be a suitable candidate for use in livestock and poultry breeding.

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鸡盲肠粪肠球菌AQ10的益生菌特性及功能特性研究

乳酸菌被认为是最安全的抗生素替代品，在畜牧业中有着广泛的应用。粪肠球菌是鸡肠道菌群的主要组成部分；众所周知，它具有调节肠道微生物平衡和益生菌功能的能力。本研究从鸡盲肠中分离出粪肠杆菌AQ10菌株，采用全基因组测序和表型分析相结合的方法研究其益生菌特性。全基因组测序结果显示，粪肠球菌AQ10基因组长度为2.98 Mbp，鸟嘌呤-胞嘧啶平均含量为38.12%，包含2832个蛋白编码基因。利用基因组挖掘工具鉴定抗菌化合物基因簇。此外，粪肠杆菌AQ10在体内表现出益生菌特性和体外抗菌作用，有机酸可能有助于其抗肺炎克雷伯菌活性。代谢组学分析表明，粪肠杆菌AQ10上清液含有7种有机酸。综上所述，粪肠杆菌AQ10具有显著的益生菌潜力，可能是畜禽养殖中应用的合适候选菌。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Letters in Applied Microbiology 工程技术-生物工程与应用微生物

CiteScore

4.40

自引率

4.20%

发文量

225

审稿时长

3.3 months

期刊介绍： Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.