Genome Analysis of a Polysaccharide-Degrading Bacterium Microbulbifer sp. HZ11 and Degradation of Alginate.

IF 4.9 2区医学 Q1 CHEMISTRY, MEDICINAL

Marine Drugs Pub Date : 2024-12-18 DOI:10.3390/md22120569

Xiao Liu, Wentao Zhao, Yan Li, Zhongliang Sun, Chang Lu, Liqin Sun

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引用次数: 0

Abstract

Marine bacteria are crucial sources of alginate lyases, which play an essential role in alginate oligosaccharide (AOS) production. This study reports the biochemical characteristics of a new species of the Microbulbifer genus, Microbulbifer sp. HZ11. The strain HZ11 is Gram-negative, aerobic, flagellate-free, and rod-shaped. The genome of strain HZ11 is a 4,248,867 bp circular chromosome with an average GC content of 56.68%. HZ11 can degrade alginate and other polysaccharides. The carbohydrate-active enzyme (CAZyme) genes account for 4.57% of the total protein-coding genes of HZ11. Its alginate metabolism process is consistent with the characteristics of the polysaccharide utilization locus (PUL) system. The alginate lyase produced by strain HZ11 showed the highest activity at 50 °C, pH 8.5, and 0.1 M NaCl. The substrate preference was as follows: sodium alginate > poly mannuronic acid > poly guluronic acid. The thin layer chromatography (TLC) results revealed that the main enzymatic degradation products were monosaccharides or AOSs with a degree of polymerization (DP) of 2-3. These results help clarify the metabolism and utilization mechanism of alginate by marine bacteria and provide a theoretical reference for its application in the degradation of alginate and other polysaccharides.

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多糖降解细菌Microbulbifer sp. HZ11的基因组分析与海藻酸盐的降解。

海洋细菌是海藻酸解酶的重要来源，海藻酸解酶在海藻酸寡糖（AOS）的生产中起着至关重要的作用。本文报道了一种名为Microbulbifer sp. HZ11的小球茎属植物的生物化学特性。菌株HZ11为革兰氏阴性，需氧，无鞭毛，杆状。菌株HZ11基因组为4,248,867 bp环状染色体，平均GC含量为56.68%。HZ11可以降解海藻酸盐和其他多糖。糖活性酶（CAZyme）基因占HZ11蛋白编码基因总数的4.57%。其藻酸盐代谢过程符合多糖利用位点（PUL）系统的特点。菌株HZ11产的海藻酸解酶在50℃、pH 8.5、0.1 M NaCl条件下活性最高。底物偏好为：海藻酸钠、聚甘露醛酸、聚古鲁醛酸。薄层色谱（TLC）结果表明，酶解产物主要为单糖或aos，聚合度（DP）为2 ~ 3。这些结果有助于阐明海洋细菌对海藻酸盐的代谢和利用机制，并为其在海藻酸盐和其他多糖降解中的应用提供理论参考。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Marine Drugs 医学-医药化学

CiteScore

9.60

自引率

14.80%

发文量

671

审稿时长

1 months

期刊介绍： Marine Drugs (ISSN 1660-3397) publishes reviews, regular research papers and short notes on the research, development and production of drugs from the sea. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible, particularly synthetic procedures and characterization information for bioactive compounds. There is no restriction on the length of the experimental section.