Microscopic Observation and Physicochemical Analysis Reveal the Microstructure and Chemical Composition of Abelmoschus manihot Flower and Its Potential Value in Anti-Caries Applications.

IF 2 3区工程技术 Q2 ANATOMY & MORPHOLOGY

Microscopy Research and Technique Pub Date : 2024-12-26 DOI:10.1002/jemt.24782

Zenghong Sun, Linyang Wang, Pin Chen, Shuge Tian, Yan Chen

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引用次数: 0

Abstract

Abelmoschus manihot flower (AMF), commonly cultivated in Southern China, is homology of medicine and food. In this study, microscopic observation revealed the microstructure of AMF, including upper epidermal cells and nonglandular hairs that play roles in defense and water management. Physicochemical analyses indicated that AMF powder exhibits weak acidity and low moisture content, suggesting its stability. Further fluorescence and spectroscopic analyses identified a diverse array of chemical constituents in AMF, including various flavonoids. Using ultra high performance liquid chromatography^_quadrupole exactive hybrid mass spectrometer (UHPLC-Q Exactive HFX) technology, a total of 407 compounds were identified in AMF, 79 of which were found to have a relative content exceeding 0.1%. A network pharmacological analysis identified 31 potential active components and delineated 10 core targets associated with dental caries. Molecular docking studies demonstrated that the selected active components exhibited strong binding affinities with the aforementioned core targets, particularly flavonoids such as quercetin and morin. In conclusion, this study provides a comprehensive analysis of AMF's microstructure and chemical composition, establishing a scientific foundation for its applications in the pharmaceutical and food industries, as well as in oral health for caries prevention, while also exploring its potential mechanisms of action.

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显微观察和理化分析揭示了马尼芒花的显微结构、化学成分及其潜在的防龋应用价值。

马尼香（Abelmoschus manihot flower， AMF）是华南地区常见的一种药食同源植物。在本研究中，显微镜观察揭示了AMF的微观结构，包括在防御和水分管理中发挥作用的上表皮细胞和非腺体毛。理化分析表明，AMF粉体具有弱酸性和低含水量的特点，具有较好的稳定性。进一步的荧光和光谱分析确定了AMF中的多种化学成分，包括各种类黄酮。采用超高效液相色谱-四极柱萃取混合质谱仪（UHPLC-Q exactive HFX）技术，共鉴定出407个化合物，其中79个化合物的相对含量超过0.1%。网络药理学分析确定了31种潜在的活性成分，并描绘了10个与龋齿相关的核心靶点。分子对接研究表明，所选择的活性成分与上述核心靶点具有较强的结合亲和力，特别是与槲皮素、桑里素等黄酮类化合物结合。综上所述，本研究全面分析了AMF的微观结构和化学成分，为其在制药和食品工业、口腔卫生预防龋齿方面的应用奠定了科学基础，并探讨了其潜在的作用机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microscopy Research and Technique 医学-解剖学与形态学

CiteScore

5.30

自引率

20.00%

发文量

233

审稿时长

4.7 months

期刊介绍： Microscopy Research and Technique (MRT) publishes articles on all aspects of advanced microscopy original architecture and methodologies with applications in the biological, clinical, chemical, and materials sciences. Original basic and applied research as well as technical papers dealing with the various subsets of microscopy are encouraged. MRT is the right form for those developing new microscopy methods or using the microscope to answer key questions in basic and applied research.