Using Serum Metabolomic Signatures to Investigate Effects of Acupuncture on Pain-Fatigue-Sleep Disturbance in Breast Cancer Survivors.

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Metabolites Pub Date : 2024-12-10 DOI:10.3390/metabo14120698

Hongjin Li, Ardith Z Doorenbos, Yinglin Xia, Jun Sun, Hannah Choi, Richard E Harris, Shuang Gao, Katy Sullivan, Judith M Schlaeger

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引用次数: 0

Abstract

Background/objectives: Acupuncture is an efficacious integrative therapy for treating pain, fatigue, and sleep disturbance (the psychoneurological symptom cluster) in breast cancer survivors. However, the mechanisms underlying its effects remain unclear, and related metabolomics studies are limited. This study aimed to examine serum metabolite changes after acupuncture and their relationships to symptom improvement.

Methods: Forty-two breast cancer survivors experiencing pain, fatigue, and sleep disturbance participated in a single-arm acupuncture trial. They received a 10-session acupuncture intervention over 5 weeks. Fasting blood samples and symptom surveys were collected before and after the acupuncture intervention, and untargeted metabolomics profiling was conducted on serum samples. Mixed-effects models adjusting for covariates (age, race, body mass index, and antidepressant use) were applied for analysis.

Results: After acupuncture, there was a significant reduction in the psychoneurological symptom cluster (mean reduction = -6.2, p < 0.001).Bonferroni correction was applied to 40 independent metabolite clusters (α = 0.00125); cysteine-glutathione disulfide (p = 0.0006) significantly increased, and retinal (p = 0.0002) and cis-urocanate (p = 0.0005) were significantly decreased. Dimethyl sulfone (p = 0.00139) showed a trend towards reduction after acupuncture and its change (p = 0.04, β =1.97) was positively associated with reduction in the psychoneurological symptom cluster. Also, increased lauroylcarnitine (p = 0.0009) and decreased cytosine (p = 0.0008) can modulate the therapeutic effects of acupuncture.

Conclusions: Acupuncture demonstrates beneficial effects on the psychoneurological symptom cluster in breast cancer survivors. Dimethyl sulfone may be a promising mediator in the relationship between acupuncture and psychoneurological symptoms, while acylcarnitine metabolism may modulate the therapeutic effect of acupuncture.

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利用血清代谢组学特征研究针刺对乳腺癌幸存者疼痛-疲劳-睡眠障碍的影响。

背景/目的：针灸是治疗乳腺癌幸存者疼痛、疲劳和睡眠障碍（精神神经症状群）的有效综合疗法。然而，其作用机制尚不清楚，相关代谢组学研究有限。本研究旨在探讨针刺后血清代谢物的变化及其与症状改善的关系。方法：42名经历疼痛、疲劳和睡眠障碍的乳腺癌幸存者参加了一项单臂针灸试验。他们在5周内接受了10次针灸干预。针刺干预前后采集空腹血样和症状调查，对血清样本进行非靶向代谢组学分析。采用调整协变量（年龄、种族、体重指数和抗抑郁药使用）的混合效应模型进行分析。结果：针刺治疗后，精神神经症状群明显减少（平均减少= -6.2,p < 0.001）。对40个独立代谢物簇进行Bonferroni校正（α = 0.00125）；半胱氨酸-谷胱甘肽二硫（p = 0.0006）显著升高，视网膜（p = 0.0002）和顺性尿酸（p = 0.0005）显著降低。针刺后二甲基砜（p = 0.00139）有降低的趋势，其变化（p = 0.04, β =1.97）与精神神经症状群的减轻呈正相关。月桂肉碱升高（p = 0.0009）和胞嘧啶降低（p = 0.0008）可调节针刺治疗效果。结论：针灸对乳腺癌幸存者的精神神经症状群有有益的影响。二甲基砜可能是针刺与神经心理症状关系的一个有希望的中介，而酰基肉碱代谢可能调节针刺的治疗效果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Metabolites Biochemistry, Genetics and Molecular Biology-Molecular Biology

CiteScore

5.70

自引率

7.30%

发文量

1070

审稿时长

17.17 days

期刊介绍： Metabolites (ISSN 2218-1989) is an international, peer-reviewed open access journal of metabolism and metabolomics. Metabolites publishes original research articles and review articles in all molecular aspects of metabolism relevant to the fields of metabolomics, metabolic biochemistry, computational and systems biology, biotechnology and medicine, with a particular focus on the biological roles of metabolites and small molecule biomarkers. Metabolites encourages scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on article length. Sufficient experimental details must be provided to enable the results to be accurately reproduced. Electronic material representing additional figures, materials and methods explanation, or supporting results and evidence can be submitted with the main manuscript as supplementary material.