Bacteriological profile, antibiotic susceptibility, and biofilm formation in children with chronic suppurative otitis media

IF 1.2 4区医学 Q3 OTORHINOLARYNGOLOGY

International journal of pediatric otorhinolaryngology Pub Date : 2025-01-01 DOI:10.1016/j.ijporl.2024.112208

Aza Bahadeen Taha

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引用次数: 0

Abstract

Background

Chronic suppurative otitis media is predominantly caused by aerobic bacterial infections, complicated by antibiotic-resistant strains and biofilm formation. This study aims to identify the aerobic bacterial pathogens in chronic suppurative otitis media among children and assess their antibiotic susceptibility patterns. The potential link between biofilm formation and antibiotic resistance is also evaluated.

Methods

A cross-sectional study was conducted on 457 children with chronic suppurative otitis media. Middle ear discharge samples were collected and aerobic bacteria were isolated and identified using standard microbiological methods. Antibiotic susceptibility was determined by the agar dilution method, and biofilm formation was assessed using the microtiter plate assay.

Results

Of the 457 cases, 89.72 % were monomicrobial infections. The most prevalent Gram-negative bacterium was Pseudomonas aeruginosa (35.71 %), while Staphylococcus aureus (26.27 %) was the leading Gram-positive pathogen. Pseudomonas aeruginosa demonstrated high resistance, with 96.77 % resistant to cefuroxime and 92.26 % to amoxicillin/clavulanic acid. Similarly, Staphylococcus aureus showed significant resistance to ampicillin (83.33 %) and amoxicillin (78.07 %). A strong correlation (p < 0.001) was observed between biofilm formation and antibiotic resistance, with Gram-negative bacteria resisting an average of 4.24 ± 1.769 antibiotics and Gram-positive bacteria resisting 5.13 ± 1.535 antibiotics.

Conclusion

A high prevalence of antibiotic-resistant pathogens has been observed in children with chronic suppurative otitis media, with a significant association between biofilm formation and antibiotic resistance.

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慢性化脓性中耳炎儿童的细菌学特征、抗生素敏感性和生物膜形成。

背景：慢性化脓性中耳炎主要由需氧细菌感染引起，并发抗生素耐药菌株和生物膜形成。本研究旨在鉴定儿童慢性化脓性中耳炎的需氧细菌病原体并评估其抗生素敏感性模式。生物膜形成与抗生素耐药性之间的潜在联系也进行了评估。方法：对457例慢性化脓性中耳炎患儿进行横断面研究。采集中耳分泌物标本，采用标准微生物学方法分离鉴定好氧菌。用琼脂稀释法测定抗生素敏感性，用微滴板法测定生物膜形成情况。结果：457例中，89.72%为单菌感染。革兰氏阴性菌以铜绿假单胞菌为主（35.71%），革兰氏阳性菌以金黄色葡萄球菌为主（26.27%）。铜绿假单胞菌对头孢呋辛的耐药率为96.77%，对阿莫西林/克拉维酸的耐药率为92.26%。同样，金黄色葡萄球菌对氨苄西林（83.33%）和阿莫西林（78.07%）表现出明显的耐药。结论：慢性化脓性中耳炎患儿中抗生素耐药病原体的患病率较高，生物膜形成与抗生素耐药之间存在显著相关性。

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来源期刊

International journal of pediatric otorhinolaryngology 医学-耳鼻喉科学

CiteScore

3.20

自引率

6.70%

发文量

276

审稿时长

62 days

期刊介绍： The purpose of the International Journal of Pediatric Otorhinolaryngology is to concentrate and disseminate information concerning prevention, cure and care of otorhinolaryngological disorders in infants and children due to developmental, degenerative, infectious, neoplastic, traumatic, social, psychiatric and economic causes. The Journal provides a medium for clinical and basic contributions in all of the areas of pediatric otorhinolaryngology. This includes medical and surgical otology, bronchoesophagology, laryngology, rhinology, diseases of the head and neck, and disorders of communication, including voice, speech and language disorders.