Two cases of fatal insulin homicide resolved using combined immunohistochemistry and immunofluorescence techniques.

IF 1.5 4区 医学 Q2 MEDICINE, LEGAL
Yiling Li, Zhonghao Yu, Yuhao Yuan, Jiaxin Zhang, Shuquan Zhao, Yijie Duan, Yiwu Zhou
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Abstract

Diabetes is a prevalent disease in modern society. Since the development and widespread use of synthetic insulin, its therapeutic application has become globally recognized for managing diabetes. However, excessive administration of insulin may lead to fatal outcomes. The rapid pharmacokinetics of insulin in the human body, combined with the lack of distinct pathological changes, makes the forensic identification of fatal insulin overdose challenging. In this study, we present two cases of fatal insulin overdose, one with a postmortem interval of up to 28 days. Postmortem specimens were collected and analyzed. Immunohistochemical and immunofluorescence assays revealed the consistent distribution of insulin and protamine at the injection sites in both cases. Insulin and protamine particles were observed in the epidermis, epidermal cells, adipocyte membranes, inter-adipocyte spaces, inflammatory cells, and glandular structures. Toxicological analyses confirmed the presence of insulin in the injected skin, subcutaneous fat, blood, urine, vitreous humor, and bile. This study provides valuable insights and guidelines for the forensic identification of insulin overdose cases.

采用免疫组织化学和免疫荧光联合技术解决了两例致死性胰岛素凶杀。
糖尿病是现代社会的一种普遍疾病。由于合成胰岛素的发展和广泛使用,其治疗应用已成为全球公认的糖尿病管理。然而,过量使用胰岛素可能导致致命的后果。胰岛素在人体内的快速药代动力学,加上缺乏明显的病理变化,使得致命的胰岛素过量的法医鉴定具有挑战性。在本研究中,我们报告了两例致死性胰岛素过量,其中一例死后间隔长达28天。采集尸体标本并进行分析。免疫组织化学和免疫荧光分析显示胰岛素和鱼精蛋白在注射部位的分布一致。在表皮、表皮细胞、脂肪细胞膜、脂肪细胞间隙、炎症细胞和腺体结构中可见胰岛素和鱼精蛋白颗粒。毒理学分析证实胰岛素存在于注射的皮肤、皮下脂肪、血液、尿液、玻璃体和胆汁中。本研究为胰岛素过量病例的法医鉴定提供了有价值的见解和指导方针。
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来源期刊
Forensic Science, Medicine and Pathology
Forensic Science, Medicine and Pathology MEDICINE, LEGAL-PATHOLOGY
CiteScore
3.90
自引率
5.60%
发文量
114
审稿时长
6-12 weeks
期刊介绍: Forensic Science, Medicine and Pathology encompasses all aspects of modern day forensics, equally applying to children or adults, either living or the deceased. This includes forensic science, medicine, nursing, and pathology, as well as toxicology, human identification, mass disasters/mass war graves, profiling, imaging, policing, wound assessment, sexual assault, anthropology, archeology, forensic search, entomology, botany, biology, veterinary pathology, and DNA. Forensic Science, Medicine, and Pathology presents a balance of forensic research and reviews from around the world to reflect modern advances through peer-reviewed papers, short communications, meeting proceedings and case reports.
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