Antihyperglycemic activity and bioguided isolation of phenolic compounds with antioxidant and cytotoxic properties from Syzygium malaccense leaves

IF 2.5 3区 医学 Q3 CHEMISTRY, MEDICINAL
Tatiane Luiza Cadorin Oldoni , Cleidiane da Silva , Thariane Carvalho Bicas , Bruno Ramalho Bresolin Ayres , Eloisa Regina Zanchet , Filomena Marafon , Alana Patrícia da Silva , Solange Teresinha Carpes , Margarete Dulce Bagatini , Jociani Ascari , Gustavo Thomé
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Abstract

This study investigated the antihyperglycemic potential of a hydroalcoholic extract from Syzygium malaccense leaves (E-SM) and isolate phenolic compounds with antioxidant and cytotoxic activities through a bioguided assay. The aim was to explore the therapeutic properties of S. malaccense in managing hyperglycemia and oxidative stress-related conditions. E-SM was prepared using ethanol-water (40:60 v/v) and purified through a solid-liquid procedure with increasing polarity solvents, yielding the acetone fraction (AceFr). Previous studies showed Ace-Fr had potent antioxidant and cytotoxic properties. Chromatographic techniques further purified AceFr, producing three bioactive subfractions (SF-1, SF-2, and SF-3). Flavonoids myricitrin (C1) and a mixture of quercitrin and mearnsitrin (M1) were isolated from SF-2 and SF-3 using TLC preparative. HPLC-DAD quantified phenolic compounds, including myricitrin, gallic acid, myricetin, and quercetin. HPLC-ESI-QTOF-MS/MS analysis identified ten compounds, primarily flavonols quercetin and myricetin and their glycosides. SF-1 exhibited a 30 % reduction in cell viability in cutaneous melanoma cells at 100 and 250 mg L−1, while SF-2 and SF-3 displayed enhanced antioxidant potential and reduced cancer cell locomotion proportional to concentration. In diabetic rats treated with E-SM (400 mg/kg), kidney tissue showed restored catalase activity similar to the control group, with consistent NPSH levels. Increased GST activity indicated tissue protection, while lower MDA levels suggested reduced lipid peroxidation across tissues. Our results indicate that three phenolic compounds with high antioxidant activity were isolated, and the chemical composition of the E-SM extract, rich in glycosylated flavonoids, could be related to its antihyperglycemic action.

Abstract Image

丁香叶中具有抗氧化和细胞毒性的酚类化合物的抗高血糖活性及生物引导分离。
本研究通过生物引导实验研究了黑荆荆叶(E-SM)水醇提取物的降糖潜能,并分离出具有抗氧化和细胞毒活性的酚类化合物。目的是探索黑螺旋藻在管理高血糖和氧化应激相关条件下的治疗特性。E-SM是用乙醇-水(40:60 v/v)制备的,通过固液法纯化,得到丙酮馏分(AceFr)。先前的研究表明,Ace-Fr具有有效的抗氧化和细胞毒性。色谱技术进一步纯化AceFr,得到三种生物活性亚组分(SF-1、SF-2和SF-3)。用薄层色谱法从SF-2和SF-3中分离得到黄酮类杨梅苷(C1)和槲皮苷与梅氏苷(M1)的混合物。HPLC-DAD定量测定酚类化合物,包括杨梅素、没食子酸、杨梅素和槲皮素。HPLC-ESI-QTOF-MS/MS分析鉴定出10个化合物,主要为黄酮醇、槲皮素和杨梅素及其苷类化合物。在100和250 mg L-1浓度下,SF-1在皮肤黑色素瘤细胞中表现出30 %的细胞活力降低,而SF-2和SF-3表现出增强的抗氧化潜力,并与浓度成比例地减少癌细胞的运动。用E-SM(400 mg/kg)处理的糖尿病大鼠,肾脏组织显示恢复的过氧化氢酶活性与对照组相似,NPSH水平一致。GST活性增加表明组织保护,而MDA水平降低表明组织中脂质过氧化减少。结果表明,分离得到三种具有较高抗氧化活性的酚类化合物,其化学成分可能与其抗高血糖作用有关,其中富含糖基化黄酮类化合物。
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来源期刊
Fitoterapia
Fitoterapia 医学-药学
CiteScore
5.80
自引率
2.90%
发文量
198
审稿时长
1.5 months
期刊介绍: Fitoterapia is a Journal dedicated to medicinal plants and to bioactive natural products of plant origin. It publishes original contributions in seven major areas: 1. Characterization of active ingredients of medicinal plants 2. Development of standardization method for bioactive plant extracts and natural products 3. Identification of bioactivity in plant extracts 4. Identification of targets and mechanism of activity of plant extracts 5. Production and genomic characterization of medicinal plants biomass 6. Chemistry and biochemistry of bioactive natural products of plant origin 7. Critical reviews of the historical, clinical and legal status of medicinal plants, and accounts on topical issues.
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