SENP2 negatively regulates RIG-I/MDA5 mediated innate immunity in black carp

IF 4.1 2区农林科学 Q1 FISHERIES

Fish & shellfish immunology Pub Date : 2025-02-01 DOI:10.1016/j.fsi.2024.110097

Yixia Chen , Jun Li , Jiaxin Fu , Lili Xiao , Jixiang Chu , Wei Qin , Jun Xiao , Hao Feng

{"title":"SENP2 negatively regulates RIG-I/MDA5 mediated innate immunity in black carp","authors":"Yixia Chen , Jun Li , Jiaxin Fu , Lili Xiao , Jixiang Chu , Wei Qin , Jun Xiao , Hao Feng","doi":"10.1016/j.fsi.2024.110097","DOIUrl":null,"url":null,"abstract":"<div><div>Mammalian SUMO specific peptidase 2 (SENP2) plays vital roles in a variety of biological procedures including the immune response. However, the effects of teleost SENP2 are still mostly unexplored. In this study, the SENP2 of black carp (<em>Mylopharyngodon piceus</em>) was cloned and characterized. The open reading frame of black carp SENP2 (bcSENP2) consists of 1800 nucleotides, which encode 600 amino acids. The reporter assay results showed that over-expression of bcSENP2 alone had a weak effect on interferon (IFN) promoter transcription activity, whereas it significantly reduced bcMDA5/bcRIG-I mediated IFN promoter transcription activity. The interaction between bcSENP2 and bcMDA5 or bcRIG-I was detected by immunoprecipitation experiments. The plaque assay and qPCR results indicated that bcMDA5 or bcRIG-I mediated antiviral capacity was attenuated by bcSENP2, while knockdown of bcSENP2 led to enhanced antiviral resistance to SVCV in host cells. In addition, the expression level of bcMDA5/bcRIG-I protein was attenuated by co-expressed bcSENP2 and MG132 treatment rescued this attenuating effect. All of these data support the conclusion that bcSENP2 inhibits bcMDA5/bcRIG-I mediated antiviral signaling by enhancing ubiquitin-proteasome mediated degradation of bcMDA5/bcRIG-I in black carp.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"157 ","pages":"Article 110097"},"PeriodicalIF":4.1000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Fish & shellfish immunology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1050464824007435","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FISHERIES","Score":null,"Total":0}

引用次数: 0

Abstract

Mammalian SUMO specific peptidase 2 (SENP2) plays vital roles in a variety of biological procedures including the immune response. However, the effects of teleost SENP2 are still mostly unexplored. In this study, the SENP2 of black carp (Mylopharyngodon piceus) was cloned and characterized. The open reading frame of black carp SENP2 (bcSENP2) consists of 1800 nucleotides, which encode 600 amino acids. The reporter assay results showed that over-expression of bcSENP2 alone had a weak effect on interferon (IFN) promoter transcription activity, whereas it significantly reduced bcMDA5/bcRIG-I mediated IFN promoter transcription activity. The interaction between bcSENP2 and bcMDA5 or bcRIG-I was detected by immunoprecipitation experiments. The plaque assay and qPCR results indicated that bcMDA5 or bcRIG-I mediated antiviral capacity was attenuated by bcSENP2, while knockdown of bcSENP2 led to enhanced antiviral resistance to SVCV in host cells. In addition, the expression level of bcMDA5/bcRIG-I protein was attenuated by co-expressed bcSENP2 and MG132 treatment rescued this attenuating effect. All of these data support the conclusion that bcSENP2 inhibits bcMDA5/bcRIG-I mediated antiviral signaling by enhancing ubiquitin-proteasome mediated degradation of bcMDA5/bcRIG-I in black carp.

查看原文本刊更多论文

SENP2负调控黑鱼rig - 1 /MDA5介导的先天免疫。

哺乳动物SUMO特异性肽酶2 （SENP2）在包括免疫反应在内的多种生物过程中发挥重要作用。然而，硬骨鱼SENP2的作用大部分仍未被探索。本研究对青鱼（Mylopharyngodon piceus）的SENP2基因进行了克隆和鉴定。黑鱼SENP2 （bcSENP2）开放阅读框由1800个核苷酸组成，编码600个氨基酸。报告基因实验结果显示，单独过表达bcSENP2对干扰素（IFN）启动子转录活性的影响较弱，而过表达bcMDA5/ bcrig - 1介导的IFN启动子转录活性显著降低。通过免疫沉淀实验检测bcSENP2与bcMDA5或bcrig - 1的相互作用。斑块分析和qPCR结果表明，bcSENP2可减弱bcMDA5或bcrig - 1介导的抗病毒能力，而bcSENP2的敲低可增强宿主细胞对SVCV的抗病毒抗性。此外，通过共表达bcSENP2， bcMDA5/ bcrig - 1蛋白的表达水平被减弱，MG132处理恢复了这种减弱作用。这些数据支持bcSENP2通过增强泛素蛋白酶体介导的bcMDA5/ bcrig - 1降解来抑制黑鱼bcMDA5/ bcrig - 1介导的抗病毒信号通路的结论。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Fish & shellfish immunology 农林科学-海洋与淡水生物学

CiteScore

7.50

自引率

19.10%

发文量

750

审稿时长

68 days

期刊介绍： Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.