Evaluation of a synergistic blend of short- and medium-chain fatty acids as a dietary intervention for the effective management of Vibriosis in shrimp culture

IF 4.1 2区农林科学 Q1 FISHERIES

Fish & shellfish immunology Pub Date : 2025-02-01 DOI:10.1016/j.fsi.2024.110098

T. Sathish Kumar , B. Naveen Rajeshwar , T. Sivaramakrishnan , Sujeet Kumar , Vidya Rajendran , Shiva Kumar , Lane Pineda , Morvarid Rezvani , S. Saravanan

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引用次数: 0

Abstract

Vibriosis caused by Vibrio spp. is imposing severe havoc and adverse effects on shrimp culture. Antibiotics are the most widely used therapeutic measures against vibriosis. However, the uncontrolled use of antibiotics may spread the antibiotic residues and increase antibiotic-resistant bacteria. In this study, a product based on a synergistic blend of short- and medium-chain fatty acids (Selacid® GG, herein referred as Sel) was evaluated against vibriosis in shrimp, P. vannamei. The shrimps (n = 30 in triplicate per treatment) were fed with Sel (0.0 %, 0.1 %, 0.2 % or 0.3 %) for 14 days. The shrimp were challenged on 8th day by immersion, and the samples were collected on the 6th day post-challenge and the 14th day post-feeding. The shrimp fed with Sel (0.1 %, 0.2 %, and 0.3 %) diet showed significantly higher total haemocyte count, while the phenoloxidase activity was significantly increased in the Sel 0.2 % diet fed group. Histological analysis unveiled inflammatory responses with haemocytic infiltration, encapsulation, and granuloma in Sel 0.2 % diet-fed shrimps with less degeneration and necrosis of hepatopancreas tubules and epithelial cells. qPCR analysis of vibrio loads in hepatopancreas revealed significantly lower vibrio count in all Sel diet-fed groups and further confirmed by culture-dependent method which showed significantly lower total vibrio count in the hepatopancreas and haemolymph of Sel 0.2 % and Sel 0.3 % diet fed shrimp. Growth parameters showed significantly higher values in weight gain, specific growth rate, and survival rate in Sel 0.2 % diet-fed shrimps. Thus, the current study confirms the efficacy of Sel (0.2 %) against vibriosis by enhancing the immune and inflammatory responses, reducing vibrio load and improving the growth and survival. Hence Sel, can be used as an alternative therapeutic for managing vibriosis in shrimp aquaculture.

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评价短链和中链脂肪酸协同混合作为对虾养殖弧菌病有效管理的膳食干预。

由弧菌引起的弧菌病对对虾养殖造成了严重的破坏和不良影响。抗生素是最广泛使用的治疗弧菌病的措施。然而，不加控制地使用抗生素可能会传播抗生素残留并增加耐药细菌。在本研究中，一种基于短链和中链脂肪酸协同混合物的产品（Selacid®GG，简称Sel）对凡纳米对虾弧菌病的治疗效果进行了评估。每组30尾，每组3个重复，分别以0.0%、0.1%、0.2%和0.3%的饲料饲喂14 d。第8天浸泡攻毒，在攻毒后第6天和饲喂后第14天采集样品。添加0.1%、0.2%和0.3%硒组的对虾总血细胞计数显著高于对照组，酚氧化酶活性显著高于对照组。组织学分析显示，在sel0.2%饲料喂养的虾中，肝胰腺小管和上皮细胞变性和坏死较少，炎症反应包括血细胞浸润、包被和肉芽肿。qPCR结果显示，各组虾肝胰脏弧菌数量均显著降低，培养依赖法进一步证实，各组虾肝胰脏和血淋巴弧菌总数均显著降低。饲料中添加0.2%饲料的对虾的增重、特定生长率和存活率显著高于饲料中添加0.2%饲料的对虾。因此，本研究证实了0.2%的Sel通过增强免疫和炎症反应，减少弧菌负荷，提高生长和存活率来对抗弧菌病的疗效。因此，可以作为治疗虾类养殖弧菌病的替代疗法。

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来源期刊

Fish & shellfish immunology 农林科学-海洋与淡水生物学

CiteScore

7.50

自引率

19.10%

发文量

750

审稿时长

68 days

期刊介绍： Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.