Short-term exposure of enrofloxacin inhibits synthesis of central estradiol through secretoneurin a/aromatase B (cyp19a1b) pathway in crucian carp

IF 3.9 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology Pub Date : 2024-12-25 DOI:10.1016/j.cbpc.2024.110112

Huazhong Liu , Gen Wan , Ping Luo , Jianzhen Huang , Jiayi Wu , Zhuocheng Nie , Jiming Ruan

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Abstract

It has been reported that enrofloxacin (ENR) disrupts metabolic pathway of steroid in female crucian carp, promoting testosterone (T) synthesis through stimulating expression of luteinizing hormone (LH) and inhibiting conversion of T to estradiol (E₂) through repressing aromatase A expression. To further learn effect of ENR on steroid metabolism in fish, this work investigated effect of ENR on central E₂ synthesis and the involved mechanisms in female crucian carp through evaluating contents of T and E₂ in blood and brain, expression of secretogranin 2a (scg2a), gonadotrophin 2 β (gth 2β, namely LH) and aromatase B (cyp19a1b) genes in brain, and activation of PI3K/Akt pathway in brain of ENR exposed female crucian carp. Results revealed that ENR promoted steroid metabolism in brain of female crucian carp, stimulated synthesis of T synthesis but inhibited conversion of T to E₂ through promoting expression of scg2a and gth 2β but repressing expression of cyp19a1b, PI3K/Akt signaling pathway participated in regulating the biological process.

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短期暴露于恩诺沙星可抑制鲫鱼通过分泌神经蛋白a/芳香化酶B （cyp19a1b）途径合成中央雌二醇。

有报道称，恩诺沙星（ENR）破坏雌鲫体内类固醇代谢途径，通过刺激黄体生成素（LH）表达促进睾酮(T)合成，通过抑制芳香化酶A表达抑制T向雌二醇（E2）转化。为了进一步了解ENR对鱼类类固醇代谢的影响，本研究通过测定ENR暴露的雌性鲫鱼血液和脑中T和E2的含量、脑中分泌颗粒蛋白2a （scg2a）、促性腺激素2β（即LH）和芳香化酶B （cyp19a1b）基因的表达以及脑内PI3K/Akt通路的激活情况，探讨ENR对雌性鲫鱼中枢E2合成的影响及其作用机制。结果表明，ENR通过促进scg2a和gth 2β的表达而抑制cyp19a1b、PI3K/Akt信号通路的表达，促进雌性鲫脑内类固醇代谢，刺激T合成，抑制T向E2的转化。

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来源期刊

Comparative Biochemistry and Physiology C-toxicology & Pharmacology 医学-动物学

CiteScore

7.50

自引率

5.10%

发文量

206

审稿时长

30 days

期刊介绍： Part C: Toxicology and Pharmacology. This journal is concerned with chemical and drug action at different levels of organization, biotransformation of xenobiotics, mechanisms of toxicity, including reactive oxygen species and carcinogenesis, endocrine disruptors, natural products chemistry, and signal transduction with a molecular approach to these fields.