Short-term exposure of enrofloxacin inhibits synthesis of central estradiol through secretoneurin a/aromatase B (cyp19a1b) pathway in crucian carp.

IF 3.9 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology Pub Date : 2024-12-25 DOI:10.1016/j.cbpc.2024.110112

Huazhong Liu, Gen Wan, Ping Luo, Jianzhen Huang, Jiayi Wu, Zhuocheng Nie, Jiming Ruan

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Abstract

It has been reported that enrofloxacin (ENR) disrupts metabolic pathway of steroid in female crucian carp, promoting testosterone (T) synthesis through stimulating expression of luteinizing hormone (LH) and inhibiting conversion of T to estradiol (E₂) through repressing aromatase A expression. To further learn effect of ENR on steroid metabolism in fish, this work investigated effect of ENR on central E₂ synthesis and the involved mechanisms in female crucian carp through evaluating contents of T and E₂ in blood and brain, expression of secretogranin 2a (scg2a), gonadotrophin 2 β (gth 2β, namely LH) and aromatase B (cyp19a1b) genes in brain, and activation of PI3K/Akt pathway in brain of ENR exposed female crucian carp. Results revealed that ENR promoted steroid metabolism in brain of female crucian carp, stimulated synthesis of T synthesis but inhibited conversion of T to E₂ through promoting expression of scg2a and gth 2β but repressing expression of cyp19a1b, PI3K/Akt signaling pathway participated in regulating the biological process.

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来源期刊

Comparative Biochemistry and Physiology C-toxicology & Pharmacology 医学-动物学

CiteScore

7.50

自引率

5.10%

发文量

206

审稿时长

30 days

期刊介绍： Part C: Toxicology and Pharmacology. This journal is concerned with chemical and drug action at different levels of organization, biotransformation of xenobiotics, mechanisms of toxicity, including reactive oxygen species and carcinogenesis, endocrine disruptors, natural products chemistry, and signal transduction with a molecular approach to these fields.