{"title":"Squaraine dye as a fluorescent probe for highly sensitive detection of pyrophosphate and alkaline phosphatase.","authors":"Wenxuan Zhu, Shuhua Zhao, Bei Yu, Yucun Tao, Chaoyang Wang, Lei Shi, Xiufeng Zhang, Jing Meng, Chengqiu Wu, Chunguang Ding, Lijia Yu","doi":"10.1007/s44211-024-00697-2","DOIUrl":null,"url":null,"abstract":"<p><p>We synthesized a squaraine dye (F-0) to develop a method for detecting pyrophosphate (PPi) and alkaline phosphatase (ALP) by modulating the fluorescence of F-0. The fluorescence intensity of the F-0 system was quenched upon the addition of Cu<sup>2+</sup> ions; however, it was restored when PPi was introduced due to the formation of a complex between PPi and Cu<sup>2+</sup>. Since ALP can hydrolyze PPi, the fluorescence of the system was quenched again upon the addition of ALP. Based on these principles, we established a fluorescent probe that exhibits an \"off-on-off\" fluorescence response. The detection limits of this method for PPi and ALP were 103 nmol dm<sup>-3</sup> and 0.18 U dm<sup>-3</sup>, respectively. Moreover, this method demonstrates good selectivity and specificity and can be applied to the detection of PPi in actual samples.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8000,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Sciences","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1007/s44211-024-00697-2","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
We synthesized a squaraine dye (F-0) to develop a method for detecting pyrophosphate (PPi) and alkaline phosphatase (ALP) by modulating the fluorescence of F-0. The fluorescence intensity of the F-0 system was quenched upon the addition of Cu2+ ions; however, it was restored when PPi was introduced due to the formation of a complex between PPi and Cu2+. Since ALP can hydrolyze PPi, the fluorescence of the system was quenched again upon the addition of ALP. Based on these principles, we established a fluorescent probe that exhibits an "off-on-off" fluorescence response. The detection limits of this method for PPi and ALP were 103 nmol dm-3 and 0.18 U dm-3, respectively. Moreover, this method demonstrates good selectivity and specificity and can be applied to the detection of PPi in actual samples.
我们合成了一种方碱染料（F-0），建立了一种通过调节F-0的荧光来检测焦磷酸盐（PPi）和碱性磷酸酶（ALP）的方法。加入Cu2+后，F-0体系的荧光强度被猝灭；然而，当引入PPi时，由于PPi和Cu2+之间形成络合物，它恢复了。由于ALP可以水解PPi，因此在加入ALP后，该体系的荧光再次猝灭。基于这些原理，我们建立了一种荧光探针，表现出“off-on-off”的荧光响应。该方法对PPi和ALP的检出限分别为103 nmol dm-3和0.18 U dm-3。该方法具有良好的选择性和特异性，可应用于实际样品中PPi的检测。
期刊介绍：
Analytical Sciences is an international journal published monthly by The Japan Society for Analytical Chemistry. The journal publishes papers on all aspects of the theory and practice of analytical sciences, including fundamental and applied, inorganic and organic, wet chemical and instrumental methods.
This publication is supported in part by the Grant-in-Aid for Publication of Scientific Research Result of the Japanese Ministry of Education, Culture, Sports, Science and Technology.
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