{"title":"Establishing a Novel Animal Model of Tricuspid Regurgitation by Self-expanding Stent.","authors":"Peng Xu, Weizhong Feng, Yinhao Jiang, Haixia Xu, Junqing Zhou","doi":"10.62713/aic.3490","DOIUrl":null,"url":null,"abstract":"<p><strong>Aim: </strong>To establish a simple, safe, and reproducible animal model of tricuspid regurgitation (TR).</p><p><strong>Methods: </strong>A self-expanding stent made of nickel-titanium shape memory metal alloy was developed. Ten white pigs were randomized into an experimental group (n = 7) and a control group (n = 3). TR was induced by an interventional procedure by implanting a self-expanding stent via the internal jugular vein (IJV) to open the tricuspid valve in the experimental group. The pigs in the control group underwent the same procedure without stent implantation. Echocardiography was performed preoperatively and at 1 month and 3 months postoperatively to assess cardiac function and structure modification.</p><p><strong>Results: </strong>Echocardiography displayed enlargement of the right atrium (RA) and tricuspid annulus, as well as reduced right heart function in the experimental group. Additionally, the TR area (p < 0.01), TR/RA (p < 0.01), RA (p < 0.05), and tricuspid annulus (p < 0.01) were significantly higher at 1 month or 3 months postoperatively compared with preoperative values. Likewise, right ventricular Tei index (RVTei) (p < 0.05) was significantly increased. However, right ventricular fractional area change (RVFAC) (p < 0.05), and tricuspid annular plane systolic excursion (TAPSE) (p < 0.05) were decreased, indicating impaired right heart function. Moreover, no significant changes were noted in the body weight of white pigs. All pigs survived, validating the successful establishment of the TR model. Notably, both the success rate and the survival rate were 100% in the experimental group. However, right heart remodeling was observed in the experimental group. Finally, no significant changes were detected in the control group before and after the operation.</p><p><strong>Conclusions: </strong>Self-expanding stent implantation via IJV is a simple and easy-to-manipulate approach for constructing an animal model of TR, which is suitable for exploring right ventricular remodeling.</p>","PeriodicalId":8210,"journal":{"name":"Annali italiani di chirurgia","volume":"95 6","pages":"1261-1269"},"PeriodicalIF":0.9000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Annali italiani di chirurgia","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.62713/aic.3490","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"SURGERY","Score":null,"Total":0}
引用次数: 0
Abstract
Aim: To establish a simple, safe, and reproducible animal model of tricuspid regurgitation (TR).
Methods: A self-expanding stent made of nickel-titanium shape memory metal alloy was developed. Ten white pigs were randomized into an experimental group (n = 7) and a control group (n = 3). TR was induced by an interventional procedure by implanting a self-expanding stent via the internal jugular vein (IJV) to open the tricuspid valve in the experimental group. The pigs in the control group underwent the same procedure without stent implantation. Echocardiography was performed preoperatively and at 1 month and 3 months postoperatively to assess cardiac function and structure modification.
Results: Echocardiography displayed enlargement of the right atrium (RA) and tricuspid annulus, as well as reduced right heart function in the experimental group. Additionally, the TR area (p < 0.01), TR/RA (p < 0.01), RA (p < 0.05), and tricuspid annulus (p < 0.01) were significantly higher at 1 month or 3 months postoperatively compared with preoperative values. Likewise, right ventricular Tei index (RVTei) (p < 0.05) was significantly increased. However, right ventricular fractional area change (RVFAC) (p < 0.05), and tricuspid annular plane systolic excursion (TAPSE) (p < 0.05) were decreased, indicating impaired right heart function. Moreover, no significant changes were noted in the body weight of white pigs. All pigs survived, validating the successful establishment of the TR model. Notably, both the success rate and the survival rate were 100% in the experimental group. However, right heart remodeling was observed in the experimental group. Finally, no significant changes were detected in the control group before and after the operation.
Conclusions: Self-expanding stent implantation via IJV is a simple and easy-to-manipulate approach for constructing an animal model of TR, which is suitable for exploring right ventricular remodeling.
期刊介绍：
Annali Italiani di Chirurgia is a bimonthly journal and covers all aspects of surgery:elective, emergency and experimental surgery, as well as problems involving technology, teaching, organization and forensic medicine. The articles are published in Italian or English, though English is preferred because it facilitates the international diffusion of the journal (v.Guidelines for Authors and Norme per gli Autori). The articles published are divided into three main sections:editorials, original articles, and case reports and innovations.
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