Silver nanowire/gold nanosphere binary plasma-assembled membranes for sensitive SERS detection of homocysteine

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2024-12-26 DOI:10.1007/s00604-024-06891-3

Shirun Peng, Yujun Zheng, Wanjing Li, Minghuan Lin, Zhihong Wu, Ruiyun You, Qingqiang Lin, Yaling Wu

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Abstract

Silver nanowire (Ag NW)/gold nanosphere (Au NS) binary plasma films were prepared using plasma coupling between Ag NWs and Au NSs. The plasma films formed by combining these two noble metals showed better sensitivity for SERS detection with a minimum detection concentration of 10⁻⁸ M for R6G compared to pure Ag NWs or Au NSs. After rational optimisation of the substrate preparation process, the substrate showed good homogeneity, reproducibility and stability. We perform an indirect detection of homocysteine (Hcy) through a specific reaction of Hcy with o-phthalaldehyde (OPA). The lowest detectable concentration of Hcy was 5 × 10⁻⁹ M. The recoveries of Hcy were 94.53 ~ 103.43% with the relative standard deviations (RSDs) of 2.53%, 9.21% and 12.26%, respectively, in the spike recovery experiments. With good selectivity and accuracy for Hcy detection, this plasma film provides an idea for the detection of disease markers in serum.

Graphical abstract

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银纳米线/金纳米球二元等离子体组装膜用于灵敏的SERS检测同型半胱氨酸

利用银纳米线（Ag NW）和金纳米球（Au NS）之间的等离子体耦合制备了银纳米线/金纳米球（Au NS）二元等离子体膜。与纯银NWs或金NSs相比，这两种贵金属结合形成的等离子体膜具有更好的SERS检测灵敏度，R6G的最小检测浓度为10−8 M。通过对底物制备工艺的合理优化，制备的底物具有良好的均匀性、重复性和稳定性。我们通过Hcy与邻苯二醛（OPA）的特异性反应间接检测同型半胱氨酸（Hcy）。Hcy的最低检测浓度为5 × 10−9 m，加样回收率为94.53 ~ 103.43%，相对标准偏差（rsd）分别为2.53%、9.21%和12.26%。该血浆膜对Hcy检测具有良好的选择性和准确性，为血清中疾病标志物的检测提供了思路。图形抽象

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.