Construction of colorimetric-fluorescent dual-signal aptamer-based assay using COF-Au nanozyme and magnetic nanoparticle–based CdTe quantum dots for sensitive zearalenone determination

IF 5.3 2区 化学 Q1 CHEMISTRY, ANALYTICAL
Xi Ma, Minyi Hui, Jiayu Yuan, Zhouping Wang, Xiaoyuan Ma
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Abstract

A dual-signal aptamer-based assay utilizing colorimetric and fluorescence techniques was developed for the determination of zearalenone (ZEN). The CdTe quantum dots, serving as the fluorescent signal source, were surface-modified onto Fe3O4@SiO2 and subsequently functionalized with the aptamer. The COF-Au was modified with complementary chain, which possessed peroxide (POD)-like enzyme properties, and could catalyze the peroxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to ox TMB, resulting in the generation of colorimetric signals. The two parts were merged based on the principle of base complementary pairing, resulting in an assembled structure exhibiting a diminished fluorescence signal due to the Förster resonance energy transfer (FRET) effect. Due to the higher affinity of the aptamer towards the target, the presence of ZEN resulted in the detachment of COF-Au, leading to an increase in supernatant concentration of COF-Au proportional to ZEN concentration. Consequently, this enhanced the catalytic ability and amplified the colorimetric signal. The fluorescence of precipitation increased simultaneously with the reduction of FRET, enabling linear detection of colorimetry in the range 0.5 ~ 10,000 μg·kg−1 and fluorescence in the range 0.1 ~ 10,000 μg·kg−1, with respective detection limits of 0.36 μg·kg−1 and 0.09 μg·kg−1. The spike recovery in wheat flour and corn ranged from 93.4 to 122.0%. This technology was simple to operate and had low cost and good application prospects.

Graphical Abstract

基于COF-Au纳米酶和磁性纳米粒子CdTe量子点的比色-荧光双信号配体灵敏测定法的构建
采用比色法和荧光技术建立了玉米赤霉烯酮(ZEN)的双信号检测方法。CdTe量子点作为荧光信号源,表面修饰到Fe3O4@SiO2上,随后用适配体功能化。COF-Au用互补链修饰,具有过氧化物(POD)样酶的性质,能催化3,3 ',5,5 ' -四甲基联苯胺(TMB)过氧化为ox TMB,从而产生比色信号。这两部分基于碱基互补配对的原理被合并,由于Förster共振能量转移(FRET)效应,导致荧光信号减弱的组装结构。由于适配体对靶标具有较高的亲和力,ZEN的存在导致COF-Au脱离,导致COF-Au的上清浓度与ZEN浓度成正比增加。因此,这增强了催化能力,放大了比色信号。随着FRET的降低,沉淀的荧光同时增强,可以在0.5 ~ 10000 μg·kg - 1范围内线性检测,荧光在0.1 ~ 10000 μg·kg - 1范围内,检出限分别为0.36 μg·kg - 1和0.09 μg·kg - 1。小麦粉和玉米穗回收率为93.4 ~ 122.0%。该技术操作简单,成本低,具有良好的应用前景。图形抽象
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来源期刊
Microchimica Acta
Microchimica Acta 化学-分析化学
CiteScore
9.80
自引率
5.30%
发文量
410
审稿时长
2.7 months
期刊介绍: As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.
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