Identification of the unique beginning of the 5’-end of the RNA1 of the Iranian squash mosaic virus genome using the 5’- RACE (Rapid amplification of cDNA ends) technique

Abstract

A beneficial technique for the detection of missing 5’ and 3’ ends of the genome is RACE (Rapid Amplification of cDNA Ends). Subsequently the Squash mosaic virus has an important role in the quality and quantity of cucurbits crops and considering that it was not possible to trace the Iranian isolate of this virus with the primers designed based on the sequence of other SqMV isolates, therefore, by using the RACE method, the initial part of the genome of Iranian isolate of SqMV was identified. The results showed that the Iranian SqMV isolate has a different genomic beginning than other isolates worldwide. Therefore it can be suggested that the RACE technique can be valuable for recognizing different ends in the genome.