Barley stripe mosaic virus-induced gene silencing for functional validation of abiotic stress in barley

IF 3.9 4区生物学 Q1 GENETICS & HEREDITY

Functional & Integrative Genomics Pub Date : 2024-12-27 DOI:10.1007/s10142-024-01508-7

Tayachew Admas, Maru Wudu, Hailu Berhanie

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引用次数: 0

Abstract

The barley stripe mosaic virus (BSMV) uses its genomic RNA components (alpha, beta, and gamma) as an efficient method for studying gene functions. It is a newly developed method that utilizes gene transcript suppression to determine the role of plant genes. BSMV derived from virus induced gene silencing (VIGS) is capable of infecting various key farming crops like barley, wheat, rice, corn, and oats. Nevertheless, the growing acceptance and enhancement of BSMV-VIGS will benefit all kinds of plants. Abiotic stresses such as drought and salt are highly affecting plant growth, development, and production. BSMV-induced temporal gene knockdown is performed during particular stressful situations to determine their specific function. The quick physiological and biochemical changes aid in confirming the role of the target genes. VIGS has a significant role to improve crop genetics and breeding, despite having certain restrictions. Thus, exploring the possible solution and addressing these difficulties will enhance the technology in the continuous advancement of plant manufacturing. BSMV-mediated VIGS has become popular in functional genomics; gene function can be determined without permanent transformation. In general, BSMV-mediated VIGS will be very helpful in the ongoing effort to develop resilient crops.

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大麦条纹花叶病毒诱导的基因沉默对大麦非生物胁迫的功能验证

大麦条纹花叶病毒（BSMV）利用其基因组RNA成分（α， β和γ）作为研究基因功能的有效方法。利用基因转录抑制来确定植物基因的作用是一种新发展起来的方法。BSMV源于病毒诱导的基因沉默（VIGS），能够感染大麦、小麦、水稻、玉米和燕麦等多种主要农作物。尽管如此，BSMV-VIGS的日益普及和增强将使各种植物受益。干旱和盐等非生物胁迫严重影响植物的生长、发育和生产。bsmv诱导的时间基因敲低是在特定的压力情况下进行的，以确定它们的特定功能。快速的生理生化变化有助于确认靶基因的作用。尽管存在一定的限制，但VIGS在改善作物遗传育种方面具有重要作用。因此，探索可能的解决方案，解决这些困难，将提高技术在工厂制造的不断进步。bsmv介导的VIGS已成为功能基因组学研究的热点；基因功能可以不经过永久转化而确定。总的来说，bsmv介导的VIGS将对正在进行的开发抗逆性作物的努力非常有帮助。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Functional & Integrative Genomics 生物-遗传学

CiteScore

3.50

自引率

3.40%

发文量

审稿时长

2 months

期刊介绍： Functional & Integrative Genomics is devoted to large-scale studies of genomes and their functions, including systems analyses of biological processes. The journal will provide the research community an integrated platform where researchers can share, review and discuss their findings on important biological questions that will ultimately enable us to answer the fundamental question: How do genomes work?