Enzymatic synthesis of semi-IPNs within hydrogel-based microfluidics†

IF 4.1 2区 化学 Q2 POLYMER SCIENCE
Chen Jiao, Dietmar Appelhans, Brigitte Voit, Nico Bruns and Jens Gaitzsch
{"title":"Enzymatic synthesis of semi-IPNs within hydrogel-based microfluidics†","authors":"Chen Jiao, Dietmar Appelhans, Brigitte Voit, Nico Bruns and Jens Gaitzsch","doi":"10.1039/D4PY01259C","DOIUrl":null,"url":null,"abstract":"<p >With the goal of achieving environmentally friendly polymer synthesis strategies, enzyme-promoted polymerisation has gradually attracted people's attention. The development of hydrogel-based microfluidics provides a new carrier system for enzymatic catalysis. Here, we report a new technique for enzyme-promoted free radical polymerisation, supported on hydrogel microdots (μHDs) within a microfluidic chip. Free radical polymerisation initiated by free horseradish peroxidase (HRP) in vials confirmed the formation of poly(<em>N</em>-isopropyl acrylamide) (PNiPAAm), achieving high molecular weight (500 000 Da) in 5 min. For polymerisation in microfluidics, disulphide-bearing μHDs were mounted on a PDMS-on-glass chip. Utilising a disulphide-thiol exchange reaction, modified HRP was then captured “from the flow” through the chip, which was confirmed by fluorescence microscopy. Various polymerisation parameters were studied in the microfluidic chip, and the successful polymer formation was confirmed by copolymerisation with a fluorescent comonomer. The physical entanglement fixed the formed polymer on the μHDs, forming a structure similar to a semi-interpenetrating network (semi-IPN). Thus, this technique provides a new direct approach to achieving semi-IPNs within microfluidic chips, showcasing the versatility in which microfluidic systems can be utilised.</p>","PeriodicalId":100,"journal":{"name":"Polymer Chemistry","volume":" 6","pages":" 742-750"},"PeriodicalIF":4.1000,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/py/d4py01259c?page=search","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Polymer Chemistry","FirstCategoryId":"92","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2025/py/d4py01259c","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"POLYMER SCIENCE","Score":null,"Total":0}
引用次数: 0

Abstract

With the goal of achieving environmentally friendly polymer synthesis strategies, enzyme-promoted polymerisation has gradually attracted people's attention. The development of hydrogel-based microfluidics provides a new carrier system for enzymatic catalysis. Here, we report a new technique for enzyme-promoted free radical polymerisation, supported on hydrogel microdots (μHDs) within a microfluidic chip. Free radical polymerisation initiated by free horseradish peroxidase (HRP) in vials confirmed the formation of poly(N-isopropyl acrylamide) (PNiPAAm), achieving high molecular weight (500 000 Da) in 5 min. For polymerisation in microfluidics, disulphide-bearing μHDs were mounted on a PDMS-on-glass chip. Utilising a disulphide-thiol exchange reaction, modified HRP was then captured “from the flow” through the chip, which was confirmed by fluorescence microscopy. Various polymerisation parameters were studied in the microfluidic chip, and the successful polymer formation was confirmed by copolymerisation with a fluorescent comonomer. The physical entanglement fixed the formed polymer on the μHDs, forming a structure similar to a semi-interpenetrating network (semi-IPN). Thus, this technique provides a new direct approach to achieving semi-IPNs within microfluidic chips, showcasing the versatility in which microfluidic systems can be utilised.

Abstract Image

半ipn在水凝胶微流体中的酶促合成
以实现环境友好型聚合物合成策略为目标,酶促聚合逐渐引起人们的关注。水凝胶微流体的发展为酶催化提供了一种新的载体体系。在这里,我们报告了一种酶促进自由基聚合的新技术,支持微流控芯片内的水凝胶微点(µhd)。由游离辣根过氧化物酶(HRP)在小瓶中引发的自由基聚合证实了聚(n-异丙基丙烯酰胺)(PNiPAAm)的形成,在5分钟内获得高分子量(500,000 Da)。对于微流体中的聚合,将含二硫化物的微硬盘安装在PDMS-on-glass芯片上。利用二硫化物-硫醇交换反应,修饰过的HRP“从流过”的芯片中被捕获,荧光显微镜证实了这一点。在微流控芯片中研究了各种聚合参数,并通过与荧光共聚物的共聚证实了聚合物的成功形成。物理缠结将形成的聚合物固定在μ hd上,形成类似半互穿网络(semi-IPN)的结构。因此,该技术为在微流控芯片内实现半ipn提供了一种新的直接方法,展示了微流控系统可以利用的多功能性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Polymer Chemistry
Polymer Chemistry POLYMER SCIENCE-
CiteScore
8.60
自引率
8.70%
发文量
535
审稿时长
1.7 months
期刊介绍: Polymer Chemistry welcomes submissions in all areas of polymer science that have a strong focus on macromolecular chemistry. Manuscripts may cover a broad range of fields, yet no direct application focus is required.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信