Neuroprotective effects of Apigenin on prenatal Valproic acid-induced autism spectrum disorder in rats

IF 2 Q3 NEUROSCIENCES

IBRO Neuroscience Reports Pub Date : 2024-12-01 DOI:10.1016/j.ibneur.2024.10.003

Mitra Farbin , Anahita Hejazi , Nahid Fakhraei , Yaser Azizi , Soraya Mehrabi , Razieh Hajisoltani

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Abstract

Valproic acid (VPA) demonstrates teratogenic effects during pregnancy. Prenatal exposure to VPA may result in autism spectrum disorder (ASD) -like phenotypes. Apigenin, a natural flavonoid, has been shown to have neuroprotective impacts due to its antioxidant properties. This study aimed to investigate the protective effects of apigenin in prenatal Valproic acid-induced autism in rats. Female rats (220–240 g, 2–3 months) received a single dose of VPA (600 mg/kg, i.p.) on the 12.5th day of gestational. The male offspring were given oral apigenin (50 mg/kg, p.o.) or the vehicle for 30 days. Behavioral tests, biochemical assessments for oxidative stress markers and pro-inflammatory cytokines were performed. VPA-treated rats exhibited increased anxiety-like behavior, and repetitive behavior. Social interaction was reduced, and detection of the novel object was impaired. Also, VPA-treated rats have shown higher levels of oxidative stress malondialdehyde (MDA) and lower GPX and superoxide dismutase (SOD) levels. Furthermore, IL-6 and TNF-α increased in the prefrotalcortex decreased. On the other hand, apigenin-treated rats restored the cognitive consequences and lowered oxidative stress and inflammation in the prefrotalcortex.

Conclusion

Chronic apigenin treatment restored the behavioral and biochemical abnormalities caused by prenatal VPA exposure.

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芹菜素对产前丙戊酸诱导的自闭症谱系障碍大鼠的神经保护作用。

丙戊酸（VPA）在怀孕期间有致畸作用。产前暴露于VPA可能导致自闭症谱系障碍（ASD）样表型。芹菜素是一种天然类黄酮，由于其抗氧化特性，已被证明具有神经保护作用。本研究旨在探讨芹菜素对产前丙戊酸致自闭症大鼠的保护作用。雌性大鼠（220-240 g， 2-3月龄）在妊娠第12.5天给予单剂量VPA（600 mg/kg, i.p.）。雄性子代口服芹菜素（50 mg/kg, p.o.）或载药30 d。进行行为测试、氧化应激标志物和促炎细胞因子的生化评估。vpa处理的大鼠表现出增加的焦虑样行为和重复行为。社会互动减少，对新事物的识别能力受损。此外，vpa处理的大鼠显示出更高水平的氧化应激丙二醛（MDA）和更低的GPX和超氧化物歧化酶（SOD）水平。额前皮质IL-6、TNF-α升高，TNF-α降低。另一方面，芹菜素治疗的大鼠恢复了认知功能，降低了前额皮质的氧化应激和炎症。结论：慢性芹菜素治疗可恢复产前VPA暴露所致的行为和生化异常。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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