LncRNA DLEU1 contributes to the progression of septic myocardial dysfunction by targeting miR-381-3p.

IF 1.5 4区 医学 Q4 IMMUNOLOGY
Central European Journal of Immunology Pub Date : 2024-01-01 Epub Date: 2024-11-12 DOI:10.5114/ceji.2024.144199
Tian Tian, Na Zhang, Guoxin Hu, Rong Lu, Jian Liu
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引用次数: 0

Abstract

Introduction: Cardiac dysfunction is a common complication of sepsis. This study aimed to elucidate the regulatory effect of DLEU1 on sepsis-induced myocardial injury.

Material and methods: HL-1 cardiomyocytes were treated with lipopolysaccharide (LPS) to mimic sepsis-induced myocardial injury in vitro, and the mouse septic model was established through cecum ligation and perforation (CLP). Cell viability was evaluated using Cell Counting Kit-8 (CCK-8), while apoptosis was assessed via Annexin-V staining. Pro-inflammatory factors including tumor necrosis factor α (TNF-α), interleukin (IL)-1 β, IL-6, and oxidative stress indicators were detected by ELISA kits. Cardiac function in mice was determined using cardiac ultrasound, and myocardial indices were detected by ELISA.

Results: DLEU1 levels were up-regulated gradually in HL-1 cardiomyocytes after LPS treatment in a dose-dependent manner, along with the overactivation of inflammatory responses and oxidative stress. DLEU1 downregulation alleviated LPS-induced cell apoptosis, inflammatory response and oxidative stress. In vivo, DLEU1 knockdown improved the cardiac function of septic mice, and alleviated inflammation and oxidative stress. MiR-381-3p, acting as a competing endogenous RNA (ceRNA) of DLEU1, reversed the effects of DLEU1 in both septic cell and mouse models.

Conclusions: The results indicate that the DLEU1/miR-381-3p axis is an intrinsic regulator of myocardial injury in sepsis.

LncRNA DLEU1通过靶向miR-381-3p参与脓毒性心肌功能障碍的进展。
心功能障碍是败血症的常见并发症。本研究旨在阐明leu1对脓毒症心肌损伤的调节作用。材料与方法:采用脂多糖(LPS)对HL-1心肌细胞进行体外模拟脓毒症引起的心肌损伤,并通过盲肠结扎穿孔(CLP)建立小鼠脓毒症模型。采用细胞计数试剂盒-8 (CCK-8)检测细胞活力,Annexin-V染色检测细胞凋亡。采用ELISA试剂盒检测促炎因子包括肿瘤坏死因子α (TNF-α)、白细胞介素(IL)-1 β、IL-6及氧化应激指标。采用心脏超声法测定小鼠心功能,ELISA法检测心肌指标。结果:LPS处理后HL-1心肌细胞中DLEU1水平呈剂量依赖性逐渐上调,炎症反应和氧化应激过度激活。DLEU1下调可减轻lps诱导的细胞凋亡、炎症反应和氧化应激。在体内,敲低leu1可改善脓毒症小鼠的心功能,减轻炎症和氧化应激。MiR-381-3p作为DLEU1的竞争内源性RNA (ceRNA),在脓毒症细胞和小鼠模型中逆转了DLEU1的作用。结论:结果表明,DLEU1/miR-381-3p轴是脓毒症心肌损伤的内在调节因子。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
3.00
自引率
0.00%
发文量
17
审稿时长
6-12 weeks
期刊介绍: Central European Journal of Immunology is a English-language quarterly aimed mainly at immunologists.
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