Metabolic Mechanism, Responses, and Functions of Genes HDH1, HDH3, and GST1 of Tea (Camellia sinensis L.) to the Insecticide Thiamethoxam

IF 12.2 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Journal of Hazardous Materials Pub Date : 2024-12-25 DOI:10.1016/j.jhazmat.2024.136969

Ping Zhang, Tianyuan Yang, Yunlong Xie, Yulong Liu, Qing X. Li, Xiangwei Wu, Rimao Hua, Weiting Jiao

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引用次数: 0

Abstract

Misuse of insecticides such as thiamethoxam (TMX) not only affects the quality of tea but also leaves residues in tea. Therefore, exploring the metabolic mechanisms of TMX in tea plants can evaluate effects of pesticides on the environment and human health. Here, effects of TMX on tea plants were studied. Malondialdehyde (MDA) content reached a maximum of 12.59 nmol/g fresh weight (FW) on 1st d under X (the recommended dose: 0.015 kg a.i./ha) of TMX. Under 2 X (0.03 kg a.i./ha), the catalase, glutathione S-transferase and superoxide dismutase activity were increased by 45.0%, 55.5%, and 49.7% at 7 d respectively. Metabolomic and transcriptomic analyses revealed that TMX significantly affected amino acid metabolism, flavonoid biosynthesis and glutathione metabolism, and induced the expression of 3-hydroxyisobutyric acid dehydrogenase genes (CsHDH1 and CsHDH3) and glutathione S-transferase gene (CsGST1). The three genes were transiently expressed in Nicotiana benthamiana for the first time to verify the function of TMX degradation, with the degradation rate of 59.2%-85.3% at X. This study elucidated the response of tea plants to abiotic stress on the molecular-scale perspective, and the molecular approaches could serve as a model for the study on pesticide metabolism in plants.

SYNOPSIS

Degradation ability of CsHDH1, CsHDH3 and CsGST1 genes to thiamethoxam was verified for the first time, providing genetic resources for phytoremediation of pollutants.

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来源期刊

Journal of Hazardous Materials 工程技术-工程：环境

CiteScore

25.40

自引率

5.90%

发文量

3059

审稿时长

58 days

期刊介绍： The Journal of Hazardous Materials serves as a global platform for promoting cutting-edge research in the field of Environmental Science and Engineering. Our publication features a wide range of articles, including full-length research papers, review articles, and perspectives, with the aim of enhancing our understanding of the dangers and risks associated with various materials concerning public health and the environment. It is important to note that the term "environmental contaminants" refers specifically to substances that pose hazardous effects through contamination, while excluding those that do not have such impacts on the environment or human health. Moreover, we emphasize the distinction between wastes and hazardous materials in order to provide further clarity on the scope of the journal. We have a keen interest in exploring specific compounds and microbial agents that have adverse effects on the environment.