The rat osteoarthritis bone score for histological pathology relevant to human bone marrow lesions and pain

Daniel F. McWilliams , Mohsen Shahtaheri , Soraya Koushesh , Chitra Joseph , Peter RW. Gowler , Luting Xu , Victoria Chapman , Nidhi Sofat , David A. Walsh
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引用次数: 0

Abstract

Objectives

Histological osteochondral characteristics of inflammation, fibrosis, vascularity, cartilage islands, vessels entering cartilage, thickened trabeculae and cysts are associated with bone marrow lesions (BMLs) in human knee osteoarthritis (OA). We identified and developed a method for scoring comparable pathology in two rat OA knee pain models.

Methods

Rats (n ​= ​8–10 per group) were injected with monoiodoacetate (MIA) or saline, or underwent meniscal transection (MNX) or sham surgery. Pain behaviour (weight bearing asymmetry and mechanical hindpaw withdrawal thresholds (PWTs)) were measured and knee samples obtained. Features associated with BMLs were evaluated using haematoxylin and eosin or Safranin-O stained knee sections. Sections were scored for chondropathy, osteophytes, synovitis and with the human OA Bone Score modified for rats (rOABS). rOABS reliability was assessed with intraclass correlation coefficient (ICC), groups were compared using Mann-Whitney U-tests, and associations examined with Spearman's rho.

Results

OABS features were more prevalent in each OA pain group than in controls. rOABS displayed good inter-rater reliability (ICC ​= ​0.79). rOABS was higher in each model than controls; MIA 3.0 (2.3–4.0) vs vehicle 0.0 (0.0–0.0), and MNX 4.0 (2.3–4.8) vs sham 0.0 (0.0–0.0), each p ​< ​0.003. rOABS was associated with OA cartilage involvement (rho ​= ​0.69, p ​< ​0.001), osteophyte (rho ​= ​0.61, p ​< ​0.001) and synovial inflammation (rho ​= ​0.76, p ​< ​0.001). Higher rOABS was associated with pain behaviour: weight bearing asymmetry (rho ​= ​0.65, p ​< ​0.001) and PWT (rho ​= ​−0.47, p ​= ​0.003).

Conclusions

Subchondral pathology in rat OA models resembles human subchondral BMLs. rOABS reliably measured subchondral pathology and was associated with OA structure and pain behaviour.
大鼠骨关节炎的组织学病理评分与人类骨髓病变和疼痛有关。
目的:炎症、纤维化、血管、软骨岛、进入软骨的血管、小梁增厚和囊肿的组织学特征与人类膝关节骨性关节炎(OA)的骨髓病变(BMLs)有关。我们确定并开发了一种方法来评分两种大鼠OA膝关节疼痛模型的可比病理。方法:每组8 ~ 10只大鼠分别注射单碘乙酸酯(MIA)或生理盐水,或半月板横断(MNX)或假手术。测量疼痛行为(负重不对称和机械后爪退缩阈值(PWTs))并获得膝关节样本。使用血红素和伊红或红花素o染色的膝关节切片评估与bml相关的特征。对软骨病变、骨赘、滑膜炎和大鼠OA骨评分(rOABS)进行评分。rOABS信度采用类内相关系数(ICC)评估,组间比较采用Mann-Whitney u检验,关联检验采用Spearman’s rho。结果:与对照组相比,OA疼痛组的OABS特征更为普遍。rOABS具有良好的量表间信度(ICC = 0.79)。各模型的rOABS均高于对照组;MIA 3.0 (2.3-4.0) vs vehicle 0.0 (0.0-0.0), MNX 4.0 (2.3-4.8) vs sham 0.0(0.0-0.0),各p结论:大鼠OA模型的软骨下病理与人软骨下BMLs相似。rOABS可靠地测量软骨下病理,并与OA结构和疼痛行为相关。
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来源期刊
Osteoarthritis and cartilage open
Osteoarthritis and cartilage open Orthopedics, Sports Medicine and Rehabilitation
CiteScore
3.30
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0.00%
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