Laura Libnan Haidar, Yuheng Wang, Aaron D. Gilmour, Elmer Austria, Badwi B. Boumelhem, Naveed Aziz Khan, Arifah Anwar Fadzil, Stuart T. Fraser, Marcela M. M. Bilek and Behnam Akhavan
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引用次数: 0
Abstract
Polymeric nanoparticles surface functionalised with fluorescent molecules hold significant potential for advancing diagnostics and therapeutic delivery. Despite their promise, challenges persist in achieving robust attachment of fluorescent molecules for real-time tracking. Weak physical adsorption, pH-dependent electrostatic capture, and hydrophobic interactions often fail to achieve stable attachment of fluorescent markers. While covalent attachment offers stability, it often entails laborious multi-step wet-chemistry processes. This work demonstrates that plasma polymerised nanoparticles (PPNs) can directly and covalently attach fluorescent molecules with no need for additional interim treatment processes. For the first time, we provide evidence indicating the formation of covalent bonds between the fluorescent molecules and PPN surfaces. Two model fluorescent molecules, fluorescein isothiocyanate (FITC) and Nile blue (NB), were attached to PPNs in a one-step process. The attached molecules remained on nanoparticle surfaces even after detergent washing, as confirmed by a combination of X-ray photoelectron spectroscopy (XPS), fluorescence spectroscopy, flow cytometry, and time-of-flight secondary ion mass spectrometry (ToF-SIMS) data. The robust attachment of fluorescent molecules on PPNs ensures their stability and functionality, enhancing the potential of these fluorescently labelled nanoparticles for diagnostic, therapeutic, and imaging applications.
期刊介绍:
Journal of Materials Chemistry A, B & C cover high quality studies across all fields of materials chemistry. The journals focus on those theoretical or experimental studies that report new understanding, applications, properties and synthesis of materials. Journal of Materials Chemistry A, B & C are separated by the intended application of the material studied. Broadly, applications in energy and sustainability are of interest to Journal of Materials Chemistry A, applications in biology and medicine are of interest to Journal of Materials Chemistry B, and applications in optical, magnetic and electronic devices are of interest to Journal of Materials Chemistry C.Journal of Materials Chemistry B is a Transformative Journal and Plan S compliant. Example topic areas within the scope of Journal of Materials Chemistry B are listed below. This list is neither exhaustive nor exclusive:
Antifouling coatings
Biocompatible materials
Bioelectronics
Bioimaging
Biomimetics
Biomineralisation
Bionics
Biosensors
Diagnostics
Drug delivery
Gene delivery
Immunobiology
Nanomedicine
Regenerative medicine & Tissue engineering
Scaffolds
Soft robotics
Stem cells
Therapeutic devices