Molecular genotyping of Theileria spp. detected in horses from Corrientes City, Argentina.

IF 1.8 3区农林科学 Q2 VETERINARY SCIENCES

Veterinary Research Communications Pub Date : 2024-12-24 DOI:10.1007/s11259-024-10618-3

Alicia P Benitez-Ibalo, Valeria N Debárbora, Atilio J Mangold, Santiago Nava, Patrick S Sebastian

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Abstract

After the previous description and characterization of Theileria equi sensu stricto (Genotype A) in Argentina, the aim of the present study was to investigate the possible heterogeneity of 18 S rDNA genotypes circulating in this country. Therefore, blood samples from 18 horses from two different neighborhoods of Corrientes City, Corrientes Province, were analyzed for infection with Theileria species. Theileria DNA was detected in five samples (27.8%). Four nearly complete 18 S rDNA gene sequences were obtained and phylogenetic analyses were carried out. The maximum likelihood tree constructed in this study revealed that two different 18 S rDNA genotypes - namely Genotype A (T. equi sensu stricto) and Genotype C (T. equi sensu lato/Theileria haneyi) - are circulating in the same horse population. These results represent to first evidence of T. equi genotype heterogeneity in Argentina and also the first detection of a member of the T. haneyi/Genotype C group. Together with reports from Brazil, the results of this study reinforce the assumption that Genotypes A and C are the predominant genotypes in South America.

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阿根廷科连特斯市马中检出的马伊勒氏菌的分子基因分型。

在对阿根廷产的A基因型的描述和鉴定之后，本研究的目的是调查在阿根廷流行的18种S rDNA基因型可能的异质性。因此，对来自科伦特斯省科伦特斯市两个不同社区的18匹马的血液样本进行了分析，以确定是否感染了希氏菌。5份样品中检出菌DNA（27.8%）。获得了4个接近完整的18s rDNA基因序列，并进行了系统发育分析。本研究构建的最大似然树揭示了两种不同的18s rDNA基因型，即基因型A（狭义马马）和基因型C（狭义马马/哈尼马马）在同一马种群中流行。这些结果首次证明阿根廷马氏弓形虫基因型异质性，也是首次发现马氏弓形虫/基因型C组成员。连同来自巴西的报告，本研究的结果加强了基因型A和C是南美洲主要基因型的假设。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary Research Communications 农林科学-兽医学

CiteScore

2.50

自引率

0.00%

发文量

173

审稿时长

3 months

期刊介绍： Veterinary Research Communications publishes fully refereed research articles and topical reviews on all aspects of the veterinary sciences. Interdisciplinary articles are particularly encouraged, as are well argued reviews, even if they are somewhat controversial. The journal is an appropriate medium in which to publish new methods, newly described diseases and new pathological findings, as these are applied to animals. The material should be of international rather than local interest. As it deliberately seeks a wide coverage, Veterinary Research Communications provides its readers with a means of keeping abreast of current developments in the entire field of veterinary science.