Encapsulation of anti-VEGF nanobody into niosome nanoparticles: a novel approach to enhance circulation half life and efficacy.

IF 3 4区医学 Q2 CHEMISTRY, APPLIED

Journal of microencapsulation Pub Date : 2024-12-23 DOI:10.1080/02652048.2024.2443435

Mohsen Chiani, Raha Abedini, Reza Ahangari-Cohan, Mahdi Behdani, Seyed Mahmoud Barzi, Nastaran Mohseni, Fatemeh Kazemi-Lomedasht

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Abstract

This study aimed to encapsulate an anti-VEGF nanobody (Nb) within niosome nanoparticles (NNPs) to enhance its circulation half life. Key parameters such as encapsulation efficiency, stability, Nb release, cytotoxicity, and cell migration inhibition in HUVEC cells were evaluated, along with pharmacokinetic studies in mice. Nb-loaded NNPs (Nb-NNPs) were successfully prepared with an encapsulation efficiency of 78.3 ± 3.2% and demonstrated stability over one month. In vitro assays revealed that Nb-NNPs enhanced cytotoxicity and significantly reduced cell migration in HUVEC cells compared to free Nb (P < 0.05). Pharmacokinetic studies in mice demonstrated a dramatically reduced elimination rate constant (0.025 h^-1 vs. 0.843 h^-1) and an extended terminal half life (27.721 h vs. 0.822 h), indicating slower clearance and prolonged systemic presence. In conclusion, these findings underscore the potential of Nb-NNPs to provide sustained and potent therapeutic effects, contributing valuable insights for advancing targeted therapeutic strategies.

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将抗vegf纳米体包封到纳米粒中：一种提高循环半衰期和疗效的新方法。

本研究旨在将抗vegf纳米体（Nb）封装在纳米粒（NNPs）中，以延长其循环半衰期。对HUVEC细胞的包封效率、稳定性、Nb释放、细胞毒性和细胞迁移抑制等关键参数进行了评估，并对小鼠进行了药代动力学研究。成功制备了负载nb的NNPs (Nb-NNPs)，包封效率为78.3±3.2%，稳定性超过1个月。体外实验显示，与游离Nb相比，Nb- nnps增强了HUVEC细胞的细胞毒性，显著减少了细胞迁移（P -1比0.843 h-1），延长了末端半衰期（27.721 h比0.822 h），表明清除较慢，全身存在时间较长。总之，这些发现强调了Nb-NNPs提供持续和有效治疗效果的潜力，为推进靶向治疗策略提供了有价值的见解。

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来源期刊

Journal of microencapsulation 工程技术-工程：化工

CiteScore

6.30

自引率

2.60%

发文量

审稿时长

3 months

期刊介绍： The Journal of Microencapsulation is a well-established, peer-reviewed journal dedicated to the publication of original research findings related to the preparation, properties and uses of individually encapsulated novel small particles, as well as significant improvements to tried-and-tested techniques relevant to micro and nano particles and their use in a wide variety of industrial, engineering, pharmaceutical, biotechnology and research applications. Its scope extends beyond conventional microcapsules to all other small particulate systems such as self assembling structures that involve preparative manipulation. The journal covers: Chemistry of encapsulation materials Physics of release through the capsule wall and/or desorption from carrier Techniques of preparation, content and storage Many uses to which microcapsules are put.