Photoacoustic and fluorescence dual-modality imaging of cerebral biomarkers in Alzheimer's disease rodent model.

IF 3 3区 医学 Q2 BIOCHEMICAL RESEARCH METHODS
Journal of Biomedical Optics Pub Date : 2024-12-01 Epub Date: 2024-12-23 DOI:10.1117/1.JBO.29.12.126002
Tianqu Zhai, Wei Zhang, Chenshuo Ma, Yanhui Ma, Yannis Mantas Paulus, Enming Joseph Su, Geoffrey Murphy, Daniel A Lawrence, Xueding Wang
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引用次数: 0

Abstract

Significance: Alzheimer's disease (AD) is a predominant form of dementia that can lead to a decline in the quality of life and mortality. The understanding of the pathological changes requires monitoring of multiple cerebral biomarkers simultaneously with high resolution. Photoacoustic microscopy resolves single capillaries, allowing investigations into the most affected types of vessels. Combined with confocal fluorescence microscopy, the relationship between plaque deposition and small vessel pathology could be better understood.

Aim: We aim to introduce a dual-modality imaging system combining photoacoustic microscopy (PAM) and confocal fluorescence microscopy (CFM) to provide a comprehensive view of both cerebral cortical vessels and amyloid- β ( A β ) plaque in AD mouse model in vivo and to identify the pathological changes of these two biomarkers.

Approach: We developed a dual-modality imaging system to image both cerebral vessel structure and A β plaque on groups of mice with different ages and phenotypes. Vessel imaging is enabled by PAM, whereas A β plaque is imaged by CFM with the aid of fluorescent dye.

Results: The small vessel density in the AD group was significantly lower than in the control group, whereas the A β plaque density in the AD group was not only higher but also increased with age.

Conclusions: This dual-modality system provides a powerful platform for biomarker monitoring of AD expressing multi-dimensional pathological changes.

阿尔茨海默病啮齿动物模型脑生物标志物的光声和荧光双模成像。
意义:阿尔茨海默病(AD)是痴呆症的主要形式,可导致生活质量和死亡率下降。了解病理变化需要同时监测多种脑生物标志物,并进行高分辨率监测。光声显微镜解决单个毛细血管,允许调查最受影响的血管类型。结合共聚焦荧光显微镜,可以更好地了解斑块沉积与小血管病理的关系。目的:采用光声显微镜(PAM)和共聚焦荧光显微镜(CFM)相结合的双模成像系统,在体内全面观察AD小鼠脑皮质血管和淀粉样蛋白β (a β)斑块,并鉴定这两种生物标志物的病理变化。方法:我们开发了一种双模成像系统,对不同年龄和表型的小鼠进行脑血管结构和a β斑块的成像。血管成像是通过PAM实现的,而A β斑块是通过CFM在荧光染料的帮助下成像的。结果:AD组小血管密度明显低于对照组,而A β斑块密度随年龄增加而升高。结论:该双模态系统为表达多维病理变化的AD生物标志物监测提供了强大的平台。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
6.40
自引率
5.70%
发文量
263
审稿时长
2 months
期刊介绍: The Journal of Biomedical Optics publishes peer-reviewed papers on the use of modern optical technology for improved health care and biomedical research.
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