Optimisation and evaluation of long circulating Ru-SLNs carrier for targeting melanoma cells.

IF 3 4区医学 Q2 CHEMISTRY, APPLIED

Journal of microencapsulation Pub Date : 2024-12-24 DOI:10.1080/02652048.2024.2443436

Hitesh Kumar Dewangan, Kamal Shah, Anil Kumar Vadaga, Manisha Veer, Perwez Alam

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引用次数: 0

Abstract

The aim of study was to prepared and evaluated rutin-loaded solid-lipid-nanoparticles (Ru-SLNs) gel for treatment of melanoma cells. SLNs were prepared by ultrasonication method through optimisation and evaluated their mean-diameter, PDI, zeta-potential, morphology, entrapment-efficiency, drug-loading, interaction by FTIR, in vitro skin permeation, stability, antioxidant/MTT assay and fluorescence microscopic. Further developed Ru-SLNs was incorporated into gel and characterised their physicochemical properties, drug contents, in vitro diffusion, ex vivo permeation and retention studies in human cadaver skin. Optimised Ru-SLNs batch showed 556.4 ± 2.6 nm mean-diameter, -21.9 mV zeta-potential, 94.8 ± 04% entrapment-efficiency, 62.3 ± 29% loading, and 86.63% release after 6 hrs. MTT assay showed, Ru-SLNs have 15.37 times more effectiveness against melanoma cells, while fluorescence microscopy confirmed the cellular uptake over time. Gel based Ru-SLNs, have reduction in flux across skin, indicating a sustained release of rutin and higher retention within the deeper epidermis layer. Finally, Ru-SLNs based gel exhibited promising potential and effectively targeting to skin's epidermal layer for melanoma cells.

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靶向黑色素瘤细胞的长循环ru - sln载体的优化与评价。

研究的目的是制备和评价负载芦丁固体脂质纳米颗粒（Ru-SLNs）凝胶治疗黑色素瘤细胞。通过优化超声法制备sln，并对其平均直径、PDI、ζ电位、形貌、包封效率、载药量、FTIR相互作用、体外透皮性、稳定性、抗氧化/MTT法和荧光显微镜进行评价。将进一步开发的ru - sln掺入凝胶中，对其物理化学性质、药物含量、体外扩散、体外渗透和人体皮肤保留进行了研究。优化后的Ru-SLNs平均直径为556.4±2.6 nm，电位为-21.9 mV，包封效率为94.8±04%，负载为62.3±29%，6 h后释放率为86.63%。MTT试验显示，ru - sln对黑色素瘤细胞的有效性提高了15.37倍，而荧光显微镜证实了细胞随时间的摄取。基于凝胶的ru - sln在皮肤上的通量减少，表明芦丁的持续释放和在更深的表皮层内的更高保留率。最后，基于ru - sln的凝胶显示出良好的潜力，并有效靶向皮肤表皮层的黑色素瘤细胞。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of microencapsulation 工程技术-工程：化工

CiteScore

6.30

自引率

2.60%

发文量

审稿时长

3 months

期刊介绍： The Journal of Microencapsulation is a well-established, peer-reviewed journal dedicated to the publication of original research findings related to the preparation, properties and uses of individually encapsulated novel small particles, as well as significant improvements to tried-and-tested techniques relevant to micro and nano particles and their use in a wide variety of industrial, engineering, pharmaceutical, biotechnology and research applications. Its scope extends beyond conventional microcapsules to all other small particulate systems such as self assembling structures that involve preparative manipulation. The journal covers: Chemistry of encapsulation materials Physics of release through the capsule wall and/or desorption from carrier Techniques of preparation, content and storage Many uses to which microcapsules are put.